| Project/Area Number |
60480277
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Hematology
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| Research Institution | Gunma University |
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Principal Investigator |
MAEKAWA Tadashi Gunma University, 医学部, 教授 (80008218)
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| Project Period (FY) |
1985 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1986: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1985: ¥2,600,000 (Direct Cost: ¥2,600,000)
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| Keywords | Acute leukemia / Proliferative cycle / Cytochemistry / Elutriation separation / Cell Separation / Chemotherapeutic agent / Cytosine arabinoside |
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| Research Abstract |
The purpose of this project is to apply the centrifugal elutriation technique for the separation of blood cells and to characterize the biochemical properties of acute leukemia cells and the cells from related disorders according to the different proliferative states as well to the disordered maturation. During the 1st year of study, basic aspects of separation technique was ascertained using human materials. On the 2nd year the technique was applied to various types of human blood cells including acute leukemia cells and megaloblastic bone marrow cells. Metabolic properties, sensitivity to chemotherapeutic agents and intracellular drug metabolism were investigated. The major results obtained may be summarized as follows :
1. This method allows the cell separation on the basis of cell size with sufficient reproducibility and minimal cell damage. Repeated operation was feasible.
2. The fractionated leukemic cells roughly corresponded to the proliferative state as judged from the nucleic acid synthetic activity. Large-sized proliferating cells were consistently more sensitive to the wide concentration range of either ara-C or methotrexate (MTX).
3. Cellular uptake and subsequent conversion of MTX to the polyglutamated forms were significantly elevated in the acute lymphocytic leukemia compared with the other types.
4. MTX uptake and polyglutamate formation positively correlated the the increasing cell size. However, the proportion of polyglutamated form to whole MTX did not differ significantlt. This indicates that polyglutamate formation is a cell-cycle independent process.
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