Differentiation of a human megaryoblastic cell line (MEG-01) and its production of platelets and platelet-derived factors

Research Project

Project/Area Number	60480281
Research Category	Grant-in-Aid for General Scientific Research (B)
Allocation Type	Single-year Grants
Research Field	Hematology
Research Institution	Nagoya University
Principal Investigator	OHNO Ryuzo Nagoya University School of Medicine, 医学部, 講師 (70093002)
Co-Investigator(Kenkyū-buntansha)	SAITO Hidehiko Nagoya University School of Medicine, 医学部, 教授 (20153819) MORISHIMA Yasuo Nagoya University School of Medicine, 医学部, 医員奥村雅男名古屋大学, 医学部, 医員 OGURA Michinori Nagoya University School of Medicine, 医学部, 医員 OKUMURA Masao Nagoya University School of Medicine
Project Period (FY)	1985 – 1986
Project Status	Completed (Fiscal Year 1986)
Budget Amount *help	¥3,400,000 (Direct Cost: ¥3,400,000) Fiscal Year 1986: ¥1,000,000 (Direct Cost: ¥1,000,000) Fiscal Year 1985: ¥2,400,000 (Direct Cost: ¥2,400,000)
Keywords	Megakaryoblastic cell line / Megakaryocyte / MEG-01 / culture cell line / Coagulation factor / Protein S / <beta> -thromboglobulin / 単クローン抗体
Research Abstract	We have established a novel human megakaryoblastic cell line (MEG-01) from a patient with blast crisis of chronic myelogenous leukemia. Using this cell line, we fhave obtained following results. (1) MEG-01 cells are differenciated morphologically and phenotypically to more mature megakaryocytes by a phorbor diester, TPA. (2) MEG-01 cells possess coagulation factors <VIII> , <V> , <XII> and <XIII> as well as protein S in their cytoplasma. Protein S, one of vitamin-K-dependent coagulation factors, is produced by MEG-01. Its function and molecular weight (84,000) is the same as one in normal human plasma. Its production was enhanced by vitamin-K and suppressed by warfarin. (3) A platelet specific protein, <beta> -thromboglobulin, is present in the culture supernatant of MEG-01 cells in serum-free medium. (4) A mouse monoclonal antibody (MG-2) was produced by the hybridoma technique using MEG-01 as an immunogen. MG-2 (IgM) reacts specifically with MEG-01 cells and human normal megakaryocytes.