The effect of snake defibrinogenase on blood fibrinolytic system

Research Project

Project/Area Number	60480287
Research Category	Grant-in-Aid for General Scientific Research (B)
Allocation Type	Single-year Grants
Research Field	Hematology
Research Institution	The Japan Snake Institute
Principal Investigator	HOKAMA Yasuji (1986-1987) , 財団法人・日本蛇族学術研究所・蛇毒研究室, 主任研究員 (20120305) 金田斉 (1985) 日本蛇族学術研究所, 研究員
Co-Investigator(Kenkyū-buntansha)	KOUDA Takashi The Japan Snake Institute, An associate researcher, 蛇毒研究室, 研究員 (10170185) KAWAMURA Yoshiharu The Japan Snake Institute, The chief of Okinawa branch, 沖縄支所, 支所長 (30109855)
Project Period (FY)	1985 – 1987
Project Status	Completed (Fiscal Year 1987)
Budget Amount *help	¥4,800,000 (Direct Cost: ¥4,800,000) Fiscal Year 1987: ¥500,000 (Direct Cost: ¥500,000) Fiscal Year 1986: ¥1,100,000 (Direct Cost: ¥1,100,000) Fiscal Year 1985: ¥3,200,000 (Direct Cost: ¥3,200,000)
Keywords	Blood fibrinolytic system / Snake Venom / トロンビン様酵素
Research Abstract	Coagulant activity of the venom of Trimeresurus okinavensis was very strong compared with Trimeresurus flavoviridis, Agkistrodon b. blomhoffii and Agkistrodon b. siniticus. Two thrombin-like enzymes (TLE) were separated from Trimeresurus okinavensis venom using SP Sephadex C-25, Bio Gel P-100 and Bio Rex 70. The molecular weights of TLE-I and II were 39,000 and 45,000 respectively. The thrombin-like enzymes indicated TAME hydrolysis activity, but no platelet aggregation activity. The fibrinogen value in plasma decreased remarkably and FDP value increased markedly by the injection of TLE-II . It was suggested that this TLE-II induce fibrinolytic activity on blood.