| Project/Area Number |
60480291
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
General surgery
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| Research Institution | Shiga university of medical science school of medicine |
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Principal Investigator |
KODAMA Masashi Shiga university of medical science school of medicine, 医学部, 教授 (50079836)
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| Co-Investigator(Kenkyū-buntansha) |
TANI Tohru Shiga university of medical science school of medicine, 医学部, 助手 (20179823)
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| Project Period (FY) |
1985 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥5,500,000 (Direct Cost: ¥5,500,000)
Fiscal Year 1986: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1985: ¥3,800,000 (Direct Cost: ¥3,800,000)
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| Keywords | endotoxin / endotoxin shock / polymyxin B / lipopolysacharide / sepsis / direct hemoperfusion / septic shock / ポリミキシンB |
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| Research Abstract |
We were able to immobilize Polymyxin B through condensation or by inducing in to react with halogen. A much larger dose of Polymyxin B adhered to one gr of polystyrene fiber after the condensation reaction than it did following the halogen reaction. Fixed polymyxin B was found to be firmly attached to the fiber. The mortality of mice after injection of PMX-F treated fluid contained endotoxin, indicated that 24 % (5 of 21) of the treated group survived. Whereas 100 % (21 of 21) of the untreated group died. In ex vivo experiments, direct hemoperfusion (DHP) by PMX-F was performed for endotoxin-injected canine. Only 12.5 % (1 of 8) survived in the control group, but 83 % (10 of 12) survived in the treated group receiving DHP with PMX-F. Mortality in the treated group decreased remarkably. The results thus indicated the efficacy of PMX-F in neutralizing endotoxin.
For the clinical application, PMX-F is being estimated blood compatibility with larger column for human. And DHP using PMX-F is tried by smaller amount of heparin than that of earlier examination. DHP can be driven safely with this quantity of heparin in animal.
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