| Project/Area Number |
60480311
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Digestive surgery
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| Research Institution | Tokyo Women's Medical College |
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Principal Investigator |
SAKURAI Yasuhisa Institute of Biomedical Engineering, Tokyo Women's Medical College, 医用工学研究施設, 教授 (20010027)
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| Co-Investigator(Kenkyū-buntansha) |
KABEI Nobuyuki Institute of Biomedical Engineering,Tokyo Women's Medical College, 医用工学研究施設, 講師 (50096583)
YUI Novuhiko Institute of Biomedical Engineering, Tokyo Women's Medical College, 医用工学研究施設, 助手 (70182665)
YAMADA Noriko Institute of Biomedical Engineering, Tokyo Women's Medical College, 医用工学研究施設, 助手 (50107314)
KATAOKA Kazunori Institute of Biomedical Engineering, Tokyo Women's Medical College, 医用工学研究施設, 講師 (00130245)
OHGAWARA hisako Deabetes Center, Tokyo Women's Medical College, 糖尿病センター, 助手 (10075468)
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| Project Period (FY) |
1985 – 1987
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| Project Status |
Completed (Fiscal Year 1987)
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| Budget Amount *help |
¥6,000,000 (Direct Cost: ¥6,000,000)
Fiscal Year 1987: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1986: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1985: ¥2,900,000 (Direct Cost: ¥2,900,000)
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| Keywords | Deabet / Bio-artificial Endocrine Pancreas / Monolayer Culture / Islet Transplantation / Isograft Implantation / Xenograft Implantation / Semi-premeable Polymer Membrane / diffusion Chamber / 糖尿病治療 / ハイブリッド型人工膵臓 / Diffusion Chamber / 合成高分子膜 / 浮遊培養 |
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| Research Abstract |
Bio-artificial endocrine pancreas (Bio-AEP) containing islet cells is more capable of curing the homeostasis of diabetic patients than mechamical insulin delivery system from the long term physiological viewpoint. Islet cells separated from mouse or rat pancreas were cultured in monolayer with 3 isobuty1-1-methylxanthine (IBMX) added to medium, so as to keep ptheir viability to secrete insulin and other hormons in response to glucose concentration for a long period. Monolayer cells cultured on plastic plate should be enclosed with semipermeable polymer membrane in order to block the passing of immunoglobulins and lymphocytes inducing the host's immune reaction. At the same time polymer membrane should pass such useful substances as glucose, insulin and albumin.
An implantable diffusion chamber for Bio-AEP was developed, which consisted of a small size holder, a culture plate with monolater islet cells and polymer membranes envelopping culture plate. Bio-AEP using mouse or rat islet cells were implanted into intraperitoneal cavities of mice. In case of isograft (mouse to mouse) implantation, glucose metabolism of diabetic animal recovered to mormal state for 100 days. However, in case of xenograft islets (rat to mouse), hyperglycemia of diabetic animal was slightly relieved only for 60 days. The passage of immunological substances was suspected as the cause of insufficient function of exnograft. The semipermeable membrane envelopping culture cells is key point of diffusion chamber type Bio-AEP Synthetic nuclepore and fluoropore membrane (pore size 0.03 - 0.6 <mu>m, membrane thickness 10 - 50 <mu>m) and capsule membrane of rabbit kidney were examined on their pormability of insulin, albumin and <gamma>-globulin. The result of experiment indicated that nuclepore of 0.03 <mu>m pore size and capsule membrane of kidney were preferable to others. The further biocompatible membrane should be developed in the field of biomaterials' science.
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