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Studies on Diffusion Chamber for Bio-artificaial Endocrine Pancreas

Research Project

Project/Area Number 60480311
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Digestive surgery
Research InstitutionTokyo Women's Medical College

Principal Investigator

SAKURAI Yasuhisa  Institute of Biomedical Engineering, Tokyo Women's Medical College, 医用工学研究施設, 教授 (20010027)

Co-Investigator(Kenkyū-buntansha) KABEI Nobuyuki  Institute of Biomedical Engineering,Tokyo Women's Medical College, 医用工学研究施設, 講師 (50096583)
YUI Novuhiko  Institute of Biomedical Engineering, Tokyo Women's Medical College, 医用工学研究施設, 助手 (70182665)
YAMADA Noriko  Institute of Biomedical Engineering, Tokyo Women's Medical College, 医用工学研究施設, 助手 (50107314)
KATAOKA Kazunori  Institute of Biomedical Engineering, Tokyo Women's Medical College, 医用工学研究施設, 講師 (00130245)
OHGAWARA hisako  Deabetes Center, Tokyo Women's Medical College, 糖尿病センター, 助手 (10075468)
Project Period (FY) 1985 – 1987
Project Status Completed (Fiscal Year 1987)
Budget Amount *help
¥6,000,000 (Direct Cost: ¥6,000,000)
Fiscal Year 1987: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1986: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1985: ¥2,900,000 (Direct Cost: ¥2,900,000)
KeywordsDeabet / Bio-artificial Endocrine Pancreas / Monolayer Culture / Islet Transplantation / Isograft Implantation / Xenograft Implantation / Semi-premeable Polymer Membrane / diffusion Chamber / 糖尿病治療 / ハイブリッド型人工膵臓 / Diffusion Chamber / 合成高分子膜 / 浮遊培養
Research Abstract

Bio-artificial endocrine pancreas (Bio-AEP) containing islet cells is more capable of curing the homeostasis of diabetic patients than mechamical insulin delivery system from the long term physiological viewpoint. Islet cells separated from mouse or rat pancreas were cultured in monolayer with 3 isobuty1-1-methylxanthine (IBMX) added to medium, so as to keep ptheir viability to secrete insulin and other hormons in response to glucose concentration for a long period. Monolayer cells cultured on plastic plate should be enclosed with semipermeable polymer membrane in order to block the passing of immunoglobulins and lymphocytes inducing the host's immune reaction. At the same time polymer membrane should pass such useful substances as glucose, insulin and albumin.
An implantable diffusion chamber for Bio-AEP was developed, which consisted of a small size holder, a culture plate with monolater islet cells and polymer membranes envelopping culture plate. Bio-AEP using mouse or rat islet cells were implanted into intraperitoneal cavities of mice. In case of isograft (mouse to mouse) implantation, glucose metabolism of diabetic animal recovered to mormal state for 100 days. However, in case of xenograft islets (rat to mouse), hyperglycemia of diabetic animal was slightly relieved only for 60 days. The passage of immunological substances was suspected as the cause of insufficient function of exnograft. The semipermeable membrane envelopping culture cells is key point of diffusion chamber type Bio-AEP Synthetic nuclepore and fluoropore membrane (pore size 0.03 - 0.6 <mu>m, membrane thickness 10 - 50 <mu>m) and capsule membrane of rabbit kidney were examined on their pormability of insulin, albumin and <gamma>-globulin. The result of experiment indicated that nuclepore of 0.03 <mu>m pore size and capsule membrane of kidney were preferable to others. The further biocompatible membrane should be developed in the field of biomaterials' science.

Report

(2 results)
  • 1987 Final Research Report Summary
  • 1986 Annual Research Report
  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] 大河原久子: 糖尿病. 28. 537-542 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] H. Ohgawara: Life Support System. 3-1. 645-648 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 大河原久子: 人工臓器. 15-3. 1497-1500 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 大河原久子: 人工臓器. 15-6. 1941-1944 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 大河原久子: 医学のあゆみ. 138-2. 107-108 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 大河原久子: 移植. 22-5. 576-577 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 大河原久子: "糖尿病医学1987" 診断と治療社, 20 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] H.Ohtawara: "A Comparative Study of Insuline Selection from Pancreatic Islets Maintained in Free-floating and Monolater Culture." J. Japan Diab. Soc.28. 537-542 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] H.Ohgawara: "Successful Implantation of Cultured Allo- and Xenograft Islets with the Use of a Deffusion Chamber." Life Support System. 3-1. 645-648 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] H.Ohgawara: "Diffusion Chamber for bioartificial endocrine pancreas.(Bio-AEP)" Jpn. J. Artif. Organs. 15-3. 1497-1500 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] H.Ohgawara: "A Possibitity of Iso- and Xenograft Pancreatic Is let Implantation by Using a Deffusion Chamber." Jpn. J. Artif. Organs. 15-6. 1941-1944 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] H.Ohgawara: "Preparation of Miniture Pig Pancreatic Endocrine Cell Culture." Igaku no ayumi. 138-2. 107-108 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] H. Ohgawara: Shindan to chiryo-sha. Tonyobyogaku, 46-65 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 大河原久子: 糖尿病. 29. 1156-1157 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] 大河原久子: 人工臓器. 15. 1941-1944 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] OHGAWARA Hisako: Life Support System,.

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-31   Modified: 2016-04-21  

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