| Project/Area Number |
60480337
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Nagoya University |
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Principal Investigator |
IWATA Hisashi Dept. Orthop. Surg. Associate Prof. Nagoya University., 医学部, 助教授 (90023796)
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| Co-Investigator(Kenkyū-buntansha) |
SATO Keiji. Dept. Orthop. Surg. Assistant Prof. Nagoya University., 医学部, 講師 (20178726)
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| Project Period (FY) |
1985 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥5,500,000 (Direct Cost: ¥5,500,000)
Fiscal Year 1986: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1985: ¥4,800,000 (Direct Cost: ¥4,800,000)
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| Keywords | abnormal fibrillogenesis / cmd (cartilage matrix deficiency) mouse / cartilage proteoglycan / type <II> collagen |
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| Research Abstract |
The research includes morphological, biochemical and immunological work about the proteoglycan in articular cartilage.
Light and electron microscopic observations on the structure of epiphyseal cartilages in the cmd/cmd mice, which had genetically failed to synthesize cartilage-characteristic proteoglycan but were normal in type <II> collagen synthesis, showed apparent abnormalities of collagen fibrils: e.g. increase in the diameter, appearance of periodic banding patterns and bundle-formation of collagen fibrils. These findings suggest that cartilage-characteristic proteoglycan (Cartilage PG) normally limits the lateral growth of collagen fibrils and affects collagen fibrillogenesis in vivo.
Chondrocytes from the cmd/cmd cartilage cultured in vitro produced nodules with greatly reduced extracellular matrix. Immunofluorescence staining revealed that the nodules of mutant cells differed from the normal in lacking cartilage PG and in uneven and reduced deposition of type <II> collagen. Exogenously added cartilage PG prepared from either normal mouse cartilage or Swarm rat chondrosarcoma to the culture medium was incorporated exclusively into the extracellular matrices of the nodules, with a concurrent correction of the abnormal distribution pattern of type <II> collagen. The incorporation of cartilage PG into the matrix was disturbed by hyaluronic acid or decasaccharide.
The results indicate that the intact from of cartilage PG is required for specific incorporation into the chondrocyte nodules, and further suggest that cartilage PG plays a regulatory role in the assembly of the matrix macromolecules.
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