Anti-tumor activity in regional lymph nodes of the patients with bladder cancer

Research Project

Project/Area Number	60480359
Research Category	Grant-in-Aid for General Scientific Research (B)
Allocation Type	Single-year Grants
Research Field	Urology
Research Institution	Kochi Medical College
Principal Investigator	FUJITA Yukitoshi Kochi Medical College, Professor, 医学部, 教授 (10033060)
Co-Investigator(Kenkyū-buntansha)	WATANABE Hironobu Kochi Medical College, Asistant, 医学部, 助手 (20201220) KAMEI Yoshihiro Kochi Medical College, Lecturer, 医学部, 講師 (40145149) 杉田治高知医科大学, 医学部, 助手 (70171155)
Project Period (FY)	1985 – 1987
Project Status	Completed (Fiscal Year 1987)
Budget Amount *help	¥5,600,000 (Direct Cost: ¥5,600,000) Fiscal Year 1987: ¥900,000 (Direct Cost: ¥900,000) Fiscal Year 1986: ¥1,500,000 (Direct Cost: ¥1,500,000) Fiscal Year 1985: ¥3,200,000 (Direct Cost: ¥3,200,000)
Keywords	Anti-tumor activity / Regional lymph nodes / Natural killer activity / T-lymphocyte / NK活性 / モノクローナル抗体 / 抗腫瘍括性
Research Abstract	We investigated natural killer (NK) activity and T-lymphocyte subsets in peripheral blood and regional lymph nodes in order to examine the host's defense mechanisms against cancer. Materials were obtained from 10 patients with bladder cancer and 3 patients with benign diiseases used as control. NK activity was measured by chemiluminescense assay, a new method which is well correlated to the ^<5i>Cr-release assay, and T-lymphoctye subsets were stained with anti-Leu-2,-3, -4,-7, and -11 monoclonal antibody. The NK activity of regional lymph nodes was lower than that of peripheral blood in all 13 cases, and the percentage of Leu 7^+ and Leu 11^+ cells was in agreement with that of the NK activity detected by chemiluminescense assay. In peripheral blood, the percentage of Leu 3^+ cells was reduced in high stage patients as compared to low stage patients and controls but in regional lymph nodes it was paradoxically increased. In cancer tissue the percentage of Leu 3^+ cells was higher than that of Leu 2^+ cells and few Leu 7^+ and 11^+ cells were obserbed. The significance of these results will become clear as more cases are examined.