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Molecular mechanism of gonadotropin and prolactin release from pituitary cells

Research Project

Project/Area Number 60480367
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Obstetrics and gynecology
Research InstitutionThe University of Tokushima (1987)
Osaka University (1985-1986)

Principal Investigator

AONO Toshihiro  University of Tokushima, School of Medicine, 医学部, 教授 (50028445)

Co-Investigator(Kenkyū-buntansha) AZUMA Keijiro  University of Tokushima, School of Medicine, 医学部, 助手 (20192958)
IRAHARA Minoru  University of Tokushima, School of Medicine, 医学部, 助手 (20160070)
IKEGAMI Hiromasa  Osaka University, Medical School, 医学部, 助手 (10184409)
HIROTA Kenji  Osaka University, Medical School, 医学部, 助手 (00189888)
MIYAKE Akira  Osaka University, Medical School, 医学部, 講師 (90093468)
田坂 慶一  大阪大学, 医学部, 助手 (50155058)
堤 博久  大阪大学, 医学部, 助手 (40135710)
寺川 直樹  大阪大学, 医学部, 助手 (90135690)
Project Period (FY) 1985 – 1987
Project Status Completed (Fiscal Year 1987)
Budget Amount *help
¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 1987: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1986: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1985: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsProlactin / LH / Pituitary cells / Calcium / GH_3 cells / Protein kinase C / Phosphatidyl inositol / TRH,LH-RH / プロラクチン / PMA / 5-HETE
Research Abstract

LH-RH induces LH from anterior pituitary gland and prolactin is secreted by TRH. Bromocriptine, a dopamine agonist, is useful for the treatment of prolactinoma. This drug not only suppresses prolactin release but also reduces tumor size. The purpose of this research is to clarify the postreceptor mechanism in prolactin and LH secretion, and molecular mechanism of inhibition of prolactin release by dopamine. (METHODS) Rat anterior pituitary cells and HG_3 cell line were used for the experiment. (RESULTS) (1) Maitotoxin induced Ca^<2+> influx into the cells and prolactin release.(2) TRH stimulated-^<32>P incorporation in phosphatidyl inositol turnover. (3)Phospholipase C, Activated by calcium, dissolved phosphatidyl inositol into diacylglycerol and inocitol triphosphate, and induced prolactin release. (4) Protein kinase C in pituitary cells was activated PMA. PMA stimulated LH and prolactin secretion. TRH induced the translocation of prolactin kinase C In GH_3 cells. (5) TRH stimulated arachidonate release from the phospholipids of pituitary cells preincubated with ^3H-arachidonate. (6) Prolactin and LH was released when pituitary cells was incubated with arachidonate. (7) Products of cyclooxygenase has no effects on prolactin release, although 5-HETE and leukotriene, 5-lipoxygenase products, induced prolactin and LH secretion.(8) Dopamine inhibited prolactin release stimulated by PMA, arachidonate, phospholipase C, maitotoxin and 5-HETE. TRH- induced prolactin release was not inhibited by dopamine in GH_3 cells which has no dopamine receptor. (CONCLUSION) TRH and LH-RH stimulated prolactin and LH respectively though diacylglycerol, protein kinase and calcium, arachidonate system. Dopamine inhibit every these signals.

Report

(2 results)
  • 1987 Final Research Report Summary
  • 1986 Annual Research Report

Research Products

(33 results)

All Other

All Publications (33 results)

  • [Publications] 広田憲二, 青野敏博, 池上博雅, 門脇浩三, 谷澤修: ホルモンと臨床. 34. 365-369 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 池上博雅, 青野敏博, 小泉清, 大塚志郎, 吉本泰弘, 福井英人, 三宅侃, 谷澤治: 日本産科婦人科学会雑誌. 38. 316 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 広田憲二, 青野敏博, 池上博雅, 門脇浩三, 谷澤修: 日本内分泌学会雑誌. 62. 324 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 池上博雅, 小池浩司, 広田憲二, 門脇浩三, 青野敏博, 谷澤修: 日本内分泌学会雑誌. 62. 332 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 林田美知子, 小泉清, 小池浩司, 池上博雅, 福井英人, 青野敏博, 谷澤修: 日本内分泌学会雑誌. 62. 334 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 小泉清, 青野敏博, 谷澤修, 伊藤芳晴, 森脇要, 森浩志, 松本圭史: 日本内分泌学会雑誌. 62. 338 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 斎藤洋一, 早川徹, 最上平太郎, 小泉清, 青木敏博, 谷澤修, 松本圭史, 森浩志: 日本内分泌学会雑誌. 62. 339 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 青野敏博: 実験医学. 5(1). 61-64 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 青野敏博, 小池浩司, 三宅侃: ホルモンと臨床. 35(2). 137-141 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] H. Ikegami;K. Koike;T. Aono;H. Fukui;K. Koizumi;O. Tanizawa: Fertility and Sterility. 47. 867-869 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 池上博雅, 門脇浩三, 広田憲二, 青野敏博, 小池浩司, 谷澤修: 日本産科婦人科学会雑誌. 39. 156 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 池上博雅, 山口正明, 広田憲二, 青野敏博, 小池浩司, 谷澤修: 日本内分泌学会雑誌. 63. 407 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 広田憲二, 小池浩司, 池上博雅, 青野敏博, 門脇浩三, 谷澤修: 日本内分泌学会雑誌. 63. 515 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 広田憲二: "続生化学実験口座7" 東京化学同人, 6 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Hirota, K., Aono, T., Ikegami, H., Kadowaki, K. and Tanizawa, O.: "Secretion of gonadotropin in pituitary and protein kinase C" Clinical Endocrinology (JPN). 34. 365-369 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Ikegami,H., Aono,T., Koizumi,K., Ohtsuka,S., Yoshimoto,Y., Fukui,H., Miyake,A. and Tanizawa,O.: "The influences of operation and bromocriptine therapies before pregnancy on puerperal lactation in patients with prolactinoma" Acta Obstetrica et Gynaecologica Japonica. 38(suppl). 316- (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Hirota,K., Aono,T., Ikegami,H., Kadowaki,K. and Tanizawa,O.: "The influence of phorbol ester on LH release and GnRH receptor(s) in rat pituitary cells." Folia Endocrinologica Japonica. 62. 324- (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Ikegami,H., Koike,K., Hirota,K., Kadowaki,K., Aono,T. and Tanizawa,O.: "Intracellular mechanism of prolaetin release -interaction of C-kinase and arachidonate systems" Folia Endocrinologica Japonica. 62. 332- (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Hayashida,M., Koizumi,K., Koike,K., Ikegami,H., Fukui,H., Aono,T. and Tanizawa,O.: "Recovery of reserve activity of prolactin release in pituitary cells after operation of prolactinoma" Folia Endocrinologica Japonica. 62. 334- (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Koizumi,K., Aono,T., Tanizawa,O., Itoh,Y., Moriwaki,K., Mori,H. and Matsumoto,K.: "The Influence of bromocriptine on secretory granules of rat prolactinoma -analysis by cell fractination and morphological measurement" Folia Endocrinologica Japonica. 62. 338- (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Saitoh,Y., Hayakawa,T., Mogami,H., Koizumi,K., Aono,T., Tanizawa, O., Matsumoto,K. and Mori,H.: "The inhibitory effect of CB-154 on cells of hormone-induced rat prolactinoma -morphological analysis" Folia Endocrinologica Japonica. 62. 339- (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Aono, T.: "Gonadotropin-releasing hormone -action mechanism and clinical application" Experimental Medicine (JPN). 5. 61-64 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Aono,T., Koike,K.,and Miyake,A.: "Short feedback regulation of gonadotropin by prolactin" Clinical endocrinology (JPN). 35. 137-141 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Ikegami,H., Aono,T., Koizumi,K., Koike,K., Fukui,H. and Tanizwa,O.: "Relationship between the method of treatment for prolcatinomas and the puerperal lactation" Fertility and Steriligy. 47. 867-869 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Ikegami,H., Hirota,K., Kadowaki,K., Aono,T. and Tanizawa,O.: "The molecular mechanism and influence of dopamine on prolactin release of pituitary cells" Acta Obstetrica et Gynaecologica Japonica. 39(suppl). 156- (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Ikegami,H., Hirota,K., Koike,K., Yamaguchi,M., Aono,T. and Tanizawa,O.: "The molecular mechanism of prolactin release of pituitary cells -the influence of PMA and arachidonate on C-kinase activities" Folia Endocrinologica Japonica. 63. 407- (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Hirota,K., Ikegami,H., Kadowaki,K., Koike,K., Aono,T. and Tanizawa,O.: "The changes of C-kinase activities in prolactin release from GH_3 cells by TRH" Folia Endocrinilogica Japonica. 63. 515- (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Hirota, H.: Tokyo Kagakudozin. Zoku-Seikagakukoza vol 7, 739 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 廣田憲二,青野敏博,池上博雅,門脇浩三,谷澤修: ホルモンと臨床. 34. 365-369 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] 廣田憲二,青野敏博,池上博雅,門脇浩三,谷澤修: 日本内分泌学会雑誌. 62(4). 324 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] 池上博雅,小池浩司,廣田憲二,門脇浩三,青野敏博,谷澤修: 日本内分泌学会雑誌. 62(4). 332 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] 池上博雅,小池浩司,小泉清,福井英人,三宅侃,青野敏博,谷澤修: 日本内分泌学会雑誌. 62(9). 961 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] 廣田憲二: "続生化学実験講座" 東京化学同人, 6 (1986)

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-30   Modified: 2016-04-21  

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