Basic research of the process of degradation of rhodopsin by the retinal pigment epithelium and the clinical application.
| Project/Area Number |
60480387
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Ophthalmology
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| Research Institution | TOHOKU UNIVERSITY |
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Principal Investigator |
HARA Satoshi Tohoku University School of Medicine, 医学部, 講師 (60108537)
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| Co-Investigator(Kenkyū-buntansha) |
ISHIGURO Sei-ichi Tohoku University School of Medicine, 医学部, 講師 (20111271)
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| Project Period (FY) |
1985 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥6,400,000 (Direct Cost: ¥6,400,000)
Fiscal Year 1986: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1985: ¥5,900,000 (Direct Cost: ¥5,900,000)
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| Keywords | ELISA / opsin / retinal pigment epithelium / cathepsin D / monoclonal antibody |
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| Research Abstract |
The retinal pigment epithelium (RPE) physiologically degrades rhodopsin which is synthesized in visual cells and plays a major part of protein in the formation of rod outer segments. The purpose of this project is to clarify the regulation of degradation of opsin by in vitro and in vivo studies. We purified cathepsin D from bovine RPE, having opsin-cleaving activity. Monoclonal antibody to cathepsin D was made using mouce myeloma P6. We will show the immunocytochemical and electron immunocytochemical data at the next meeting of Society of Japanese Ophthalmology. We studied also the content and the distribution of opsin in pathological conditions. Immunoreactive opsin decreased with the duration of the retinal detachment, assayed by ELISA. Opsin cleaving enzyme in subretinal fluid may degrade opsin. C3H mice, retinal degeneration mice, was shown to have abnormal polarity in regard to the localization of opsin. Normal synthesis, transport and degradation of opsin were disturbed. The retina of the case of anterior type of persistent hyperplastic primary vitreous showed retinal dysplasia. Immunocytochemistry of opsin also revealed abnormal accumulation of opsin in visual cells. A case of partial 5q trisomy and 7q monosomy had Leber's congenital amaurosis. Chromosome 7 might play an important role in organogenesis of visual cells.
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Report
(1 results)
Research Products
(8 results)
