| Project/Area Number |
60480395
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Niigata University |
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Principal Investigator |
KOBAYASHI Shigeo School of Dentistry, Niigata University, Professor, 歯学部, 教授 (40018403)
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| Co-Investigator(Kenkyū-buntansha) |
ARAI Hideaki School of Dentistry, Niigata University, Assistant, 歯学部, 助手 (00175952)
YOSHIDA Shigemitsu School of Dentistry, Niigata University, Associate Professor, 歯学部, 助教授 (80174928)
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| Project Period (FY) |
1985 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥6,800,000 (Direct Cost: ¥6,800,000)
Fiscal Year 1986: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1985: ¥6,300,000 (Direct Cost: ¥6,300,000)
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| Keywords | Immunohistochemistry / Neurofilament protein (NFP) / S-100 protein / Dental pulp / Periodontal ligament / Human / Rat |
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| Research Abstract |
Sensory innervation in the dental tissues has been studied for years in our group with the aid of the urea silver impregnation method and the transmission electron microscope. Although the methods were useful for demonstrating the distribution and ultrastructure of nerves in the dental tissues, their origin and nature remained to be further clarified. Thus, the technique of immunohistochemistry for nervous system-specific proteins was introduced to study the morphology of neural elements. With the use of antisera against neurofilament protein (NFP) and S-100 protein which are specific for neurons and glial elements, respectively, the innervation in human teeth, rat molars, and rat periodontium was discussed. With the methods devised for fixation, decalcification and immunostaining of decalcified components. The three-dimensional constitution of nerves in the human dentin and predentin was clearly demonstrated, and the sensory mechanism of human teeth was discussed. In rat molar teeth, the NFP-positive fibers in the dentin and predentin were found to penetrate directly into the dentinal tubules. However, complex nerve fibers showing three-dimensional extension as recognized in human teeth were not observed. In the periodontal ligament of rat molars, the presence of numerous Ruffini-like corpuscles was revealed in the light microscopy. These nerve terminals are considered to function as stretch receptors in the periodontal ligament. The nerve fibers showing calcitonin gene-related peptide (CGRP) were first shown to be present in the predentin of rat molars with light and electron microscopes, and the functional significance of peptidergic neurons was discussed.
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