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Transduction mechanism of four basic taste stimuli into electric signals of taste cell

Research Project

Project/Area Number 60480402
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Functional basic dentistry
Research InstitutionNagasaki University

Principal Investigator

SATO Toshihide  Prof. Nagasaki University, 歯学部, 教授 (60013968)

Co-Investigator(Kenkyū-buntansha) OKADA Yukio  Res. Assoc. Nagasaki University, 歯学部, 助手 (60136687)
MIYAMOTO Takenori  Res. Assoc. Nagasaki University, 歯学部, 助手 (10167679)
Project Period (FY) 1985 – 1986
Project Status Completed (Fiscal Year 1986)
Budget Amount *help
¥6,500,000 (Direct Cost: ¥6,500,000)
Fiscal Year 1986: ¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 1985: ¥3,700,000 (Direct Cost: ¥3,700,000)
KeywordsFrog Tongue / Taste Cell / Four Basic Taste Stimuli / Receptor Potential / Taste Receptor Membrane / Basolateral Membrane / Ionic Permeability / 界面電位
Research Abstract

1. The purpose of this project is to clarify the mechanisms underlying the generation of receptor potential of the taste cell in response to the four taste stimuli.
2. The in situ frog tongues were used and intracellular receptor potentials were recorded from single taste cells of the fungiform papillae by inserting a 3 M KCl-filled microelectrode into the cell. As the four basic taste stimuli, 0.5 M NaCl, 1 mM HCl, 10 mM quinine-HCl (Q-HCl) and 1 M garactose were used.
3. The receptor potential for NaCl was greatly reduced by removing mono- and divalent cations from the superficial fluid over the tongue and <Na^+> from the interstitial fluid surrounding taste cells. Therefore it is concluded that the NaCl-induced receptor potential is generated by ionic currents flowing cation-selective channel of the receptor membrane and <Na^+> channel of the basolateral membrane.
4. The receptor potential for HCl was not affected by interstitial ions, but was greatly depressed by removing <Ca^(2+)> and <Na^+> in the superficial fluid and by adding furosemide to it. Even after adaptation of the tongue to water, large acid response was evoked. Therefore, the large portion of the HCl-induced response is generated by <Ca^(2+)> channel of the receptor membrane but the remaining portion is generated by the phase boundary potential.
5. The receptor potential for Q-HCl was greatly increased by reducing <Cl^-> in the superficial fluid and was reduced by removing <Na^+> and <Ca^(2+)> and adding furosemide in the interstitial fluid. Therefore, the most of the Q-HCl response is generated by a release of <Cl^-> accumulated by a <Na^+> / <Cl^-> cotransport.
6. The receptor potential for galactose was greatly reduced by removing <Cl^-> and adding DCCD in the interstitial fluid and increased by reducing a pH of superficial fluid. Thus it is suggested that the galactose response is generated by a release of <OH^-> which is related to <Cl^-> -dependent <H^+> pump.

Report

(1 results)
  • 1986 Final Research Report Summary
  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] Okada,Y.: Comp.Biochem.Physiol.81A. 247-250 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Miyamoto,T.: Jpn.J.Oral Biol.27. 389-391 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 岡田幸雄: 味と匂のシンポジウム論文集. 19. 122-125 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 宮本武典: 味と匂のシンポジウム論文集. 19. 185-188 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Okada,Y.: Jpn.J.Physiol.36. 139-150 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 岡田幸雄: 味と匂のシンポジウム論文集. 20. (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Autrum,H.: "Progress in Sensory Physiology,Vol.6" Springer-Verlag, 227 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Okada, Y.: "Artificial perfusion of frog tongue for intracellular recording of taste cell receptor potential." Comp. Biochem. Physiol.81A. 247-250 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Okada, Y.: "Role of receptor and basolateral membranes to resting potential of a frog taste cell." Proceedings of Japanese Symposium on Taste and Smell.19. 122-125 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Miyamoto, T.: "Ionic basis of salt-induced receptor potential in frog taste cells." Proceedings of Japanese Symposium on Taste and Smell.19. 185-188 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Okada, Y.: "Contribution of receptor and basolateral membranes to the resting potential of a frog taste cell." Jpn. J. Physiol.36. 139-150 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Okada, Y.: "Role of ions to receptor potential elicited by quinine in frog taste cell." Proceedings of Japanese Symposium on Taste and Smell.20. (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Autrum, H.: Springer-Verlag.Progress in Sensory Physiology, Vol.6., 1-227 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary

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Published: 1987-03-31   Modified: 2016-04-21  

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