| Project/Area Number |
60480404
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Functional basic dentistry
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| Research Institution | The Nippon Dental University |
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Principal Investigator |
MASUHARA Taizo Dept. of Dental Pharmacology, Nippon Dental Univ., at Niigata, 歯学部, 教授 (00060400)
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| Co-Investigator(Kenkyū-buntansha) |
AOKI Chihiro Dept. of Dental Pharmacology, Nippon Dental Univ., at Niigata, 新潟歯学部, 助手 (40167789)
IWABUCHI Yoshiki Dept. of Dental Pharmacology, Nippon Dental Univ., at Niigata, 新潟歯学部, 講師 (80095067)
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| Project Period (FY) |
1985 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥4,900,000 (Direct Cost: ¥4,900,000)
Fiscal Year 1986: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1985: ¥3,200,000 (Direct Cost: ¥3,200,000)
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| Keywords | rat / submandibular gland / acini / convoluted duct / submandibular saliva / salivary stimulating agent / secretable glycoprotein species / マイクロディスク電気泳動 |
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| Research Abstract |
Investigations to elucidate action sites of fourteen salivary stimulating agents on secretory functional segments of rat submandibular gland were carried out using three characteristic glycoprotein species which are electrophoretically recognized as band <I> (130KDa) and <IV> (21,5KDa) in acini and band <III> (31KDa) in convoluted tubules as a marker protein in saliva.
For this purpose, secretory behavior and electrophoretical profiles of glycoproteins in saliva elicited by various agent were observed and results obtained are as follows;
(1) All choline esters applied in this experiment showed much larger band <I> associated with small band <IV> and <III> on densitogram, and strength of secretable potency of the esters were in the order of carbachol >>bethanechol <:!=> methacholine >> acetylcholine.
(2) Pilocarpine at the dose of 0.5 to 4 mg/kg showed large band <I> like the esters and relative amount of its band increased dose-dependently, but this increase was inhibited by pretreatment
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of propranolol. (3) Adrenergic <(alpha)_1> -agonists, on the contrary, showed intense band <III> instead of band <I> in cholinergic agents, but <(alpha)_2> -agonist showed densitogram with extensive band <I> and <III> . The extend of salivation was greater in <(alpha)_1> -agonists than <(alpha)_2> -agonist. (4) Selective <(beta)_1> and <(beta)_2> adrenergic agonists showed large intense band <I> as well as the choline esters. Potency for salivation was greater in <(beta)_1> -agonist than in <(beta)_2> -agonist. (5) Administration of dopamine at the dose of 10 and 40 mg/kg showed highest band <I> , mediate band <IV> and mild band <III> on the densitogram, but in the case of 40 mg/kg of dopamine, pretreatment of propranolol presented predominant band <III> compared to band <I> and <IV> . (6) Substance P at the dose of 0.5 to 20 <mu> g/ kg showed simillar profiles to the choline esters.
These results suggest that choline esters, pilocarpine, low dose of dopamine and substance P mainly, may be, act to acini, adrenergic <(alpha)_1> -agonist and high dose of dopamine act to convoluted tubules and <(alpha)_2> -agonist act to both acini and convoluted tubules. Less
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