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Studies on the Proteins of Prism Sheath of Porcine Immature Enamel

Research Project

Project/Area Number 60480406
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Functional basic dentistry
Research InstitutionTsurumi University

Principal Investigator

SHIMIZU Masaharu  Tsurumi University, Profesor, 歯学部, 教授 (40064357)

Co-Investigator(Kenkyū-buntansha) IJIRI Hagano  Tsurumi University, Assistant, 歯学部, 助手 (50097309)
YAMAKOSI Yasuo  Tsurumi University, Assistant (20182470)
Project Period (FY) 1985 – 1987
Project Status Completed (Fiscal Year 1987)
Budget Amount *help
¥6,800,000 (Direct Cost: ¥6,800,000)
Fiscal Year 1987: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1986: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1985: ¥5,500,000 (Direct Cost: ¥5,500,000)
KeywordsTooth enamel / Prism sheath / Enamel proteins
Research Abstract

The prism sheath of porcine immature enamel consists of high molecular weight proteins(higher than 30000 daltons) and highly aggregative lower molecular weight components(in the 6000-30000 range). From these components, glycine and tyrosine rich components(MW approximately 7000) and serine and glutamic acid rich components(MW approximately 12000) were separated by gel filtration, and were purified by HPLC using C-18 reverse phase column. The results of amino acied analysis and amino acid sequencing indicated that glycine and tyrosing rich component was derived from amelogenin and it corresponded to approximately one third of the molecular length from the amino terminal of the molecule. On the other hand, as indicated below, the sequences of the first 20 residues of two serine and glutamic acid rich components were different from that of amelogenin.
1. Met-Leu-Ser-Gln-Tyr-Ser-Arg-Phe-Gly-Phe-Gly-Lys-Ser-Phe-Asn- Ser-Leu-Met-Ser-Ser-
2. Met-Lue-Ser-Gln-Tyr-Ser-Arg-Phe-Gly-Phe-Gly-Lys-Ser-Phe-Asn- Asp-Leu-Asp-Met-Ser-

Report

(2 results)
  • 1987 Final Research Report Summary
  • 1986 Annual Research Report
  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] 石井淑夫, 室淳子, 山越康雄, 清水正春: 日本化学会誌. 2064-2069 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 清水正春: "エナメル質, その形成, 構造, 組織と進化" クインテッセンス出版株式会社, 375 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Ishii, T., Muro, J., Yamakoshi, Y. and Shimizu, M.: ""Suminagashi" used in studies on monolayers" J. Chemical Society of Japan, Chemistry and Industrial Chemistry. 11. 2064-2069 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Shimizu, M.: ed. Suga, S. Quintessence Pub. Co.Enamel proteins. in Tooth Enamel, its formation, structure, composition and evolution, 139-152 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary

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Published: 1987-03-31   Modified: 2016-04-21  

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