Studies on the Proteins of Prism Sheath of Porcine Immature Enamel

Research Project

Project/Area Number	60480406
Research Category	Grant-in-Aid for General Scientific Research (B)
Allocation Type	Single-year Grants
Research Field	Functional basic dentistry
Research Institution	Tsurumi University
Principal Investigator	SHIMIZU Masaharu Tsurumi University, Profesor, 歯学部, 教授 (40064357)
Co-Investigator(Kenkyū-buntansha)	IJIRI Hagano Tsurumi University, Assistant, 歯学部, 助手 (50097309) YAMAKOSI Yasuo Tsurumi University, Assistant (20182470)
Project Period (FY)	1985 – 1987
Project Status	Completed (Fiscal Year 1987)
Budget Amount *help	¥6,800,000 (Direct Cost: ¥6,800,000) Fiscal Year 1987: ¥500,000 (Direct Cost: ¥500,000) Fiscal Year 1986: ¥800,000 (Direct Cost: ¥800,000) Fiscal Year 1985: ¥5,500,000 (Direct Cost: ¥5,500,000)
Keywords	Tooth enamel / Prism sheath / Enamel proteins
Research Abstract	The prism sheath of porcine immature enamel consists of high molecular weight proteins(higher than 30000 daltons) and highly aggregative lower molecular weight components(in the 6000-30000 range). From these components, glycine and tyrosine rich components(MW approximately 7000) and serine and glutamic acid rich components(MW approximately 12000) were separated by gel filtration, and were purified by HPLC using C-18 reverse phase column. The results of amino acied analysis and amino acid sequencing indicated that glycine and tyrosing rich component was derived from amelogenin and it corresponded to approximately one third of the molecular length from the amino terminal of the molecule. On the other hand, as indicated below, the sequences of the first 20 residues of two serine and glutamic acid rich components were different from that of amelogenin. 1. Met-Leu-Ser-Gln-Tyr-Ser-Arg-Phe-Gly-Phe-Gly-Lys-Ser-Phe-Asn- Ser-Leu-Met-Ser-Ser- 2. Met-Lue-Ser-Gln-Tyr-Ser-Arg-Phe-Gly-Phe-Gly-Lys-Ser-Phe-Asn- Asp-Leu-Asp-Met-Ser-