Effects of periodontally related microorganisms on metabolism of human fibroblasts from gingivae with various forms of periodontal disease
| Project/Area Number |
60480413
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Conservative dentistry
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| Research Institution | OKAYAMA UNIVERSITY |
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Principal Investigator |
MURAYAMA Yoji Okayama University Dental School . Professor, 歯学部, 教授 (50029972)
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| Co-Investigator(Kenkyū-buntansha) |
KINOSHITA Masahiko Okayama University Dental School Hospital . Lecturer, 歯学部附属病院, 講師 (80161537)
KURIHARA Hidemi Okayama University Dental School Hospital . Lecturer, 歯学部附属病院, 講師 (40161765)
NOMURA Yoshio Okayama University Dental School . Assistant Professor, 歯学部, 助教授 (50107075)
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| Project Period (FY) |
1985 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥6,000,000 (Direct Cost: ¥6,000,000)
Fiscal Year 1986: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1985: ¥5,400,000 (Direct Cost: ¥5,400,000)
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| Keywords | Forms of periodontal disease / Biological activities of periodontopathic bacteria / Fibroblasts from gingiva / Fibroblasts and bacterial components |
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| Research Abstract |
The purpose of this study is to evaluate the metabolism of human gingival fibroblasts from normal and various forms of periosontal disease for architectural components of periodontally related microorganisms.
Ten cell lines of the gingival fibroblast were prepared from donors with different 3 types of periodontal disease and donors with healthy disease and donors with healthy gingiva : adult periodontitis, 3 cell lines ; rapidly progressive periodontitis, 2 cell lines ; juvenile periodontitis, 1 cell line ; and normal, 2 cell lines. The cell lines were proliferated and stored in-80゜C.
Sonication extracts, sodium dodecyl sulphate extracts, and lipopolysaccharide components from the cell envelopes of Bacteroides gingivalis #381, B. asaccharolyticus ATCC 25260, Actinobacillus actinomycetemcomitans Y4 and ATCC 29523, Capnocytophaga ochracea S3, Fusobacterium nucleatum ATCC 25586.
The architectural components from a variety of periodontally related microorganisms affected dose-dependently on various metabolic functions of fibroblast : cell configuration, cell adherence, glycosaminoglycan synthesis, collagen synthesis, or DNA synthesis. A fibroblast from juvenile periodontitis and a cell line from rapidly progressive periodontitis were greatly deformed in configuration, inhibited in cell adherence, and reduced in DNA synthesis by the components of A. actinomycescomitans. These results suggest that gingival fibroblast have different response to bacterial extracts among disease types of donors.
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Report
(1 results)
Research Products
(6 results)
