• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Mechanisms of stimuli discrimination and transduction in gustatory and olfactory cells

Research Project

Project/Area Number 60480457
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Biological pharmacy
Research InstitutionHokkaido University

Principal Investigator

KURIHARA Kenzo  Faculty of Pharmaceutical Sciences, Hokkaido University Prof., 薬学部, 教授 (00016114)

Co-Investigator(Kenkyū-buntansha) KASHIWAYNAGI Makoto  Faculty of Pharmaceutical Sciences, Hokkaido University Assistant, 薬学部, 教務職員 (20169436)
MATSUOKA Ichiro  Faculty of Pharmaceutical Sciences, Hokkaido University Instructor, 薬学部, 助手 (40157269)
MIYAKE Michihisa  Faculty of Pharmaceutical Sciences, Hokkaido University Associate Prof., 薬学部, 助教授 (30133771)
Project Period (FY) 1985 – 1986
Project Status Completed (Fiscal Year 1986)
Budget Amount *help
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1986: ¥1,900,000 (Direct Cost: ¥1,900,000)
KeywordsTaste cell / Olfactory cell / Neuroblastoma / Liposome / Membrane Potential / Phase boundary potential / ニオイ
Research Abstract

The discrimination mechanisms of chemical stimuli in taste and olfactory cells were studied using taste cells, neuroblastoma and liposomes. Among various taste stimuli, bitter substances were found to adsorb on lipid layers of the membranes, which leads to generation of the receptor potentials. "Taste blind" for phenylthiocarbamide in man was examined in terms of difference in lipid composition of the receptor membranes. As for the discrimination of odorants, various mechanisms have been proposed. Many investigators have believed that there exist specific receptor protein for each odorant, but question arises whether or not animals have respective specific receptor proteins for numerous odorants. We found that neuroblastoma cells and liposomes having certain lipid compositions are depolarized by various odorants similarly to olfactory cells. Changes in the lipid composition led to changes in specificity to various odorants. The present results suggested that odorant discrimination can be explained by posturating that lipid composition of receptor membrane of each olfactory cell is different from one cell to another.
The mechanisms of changes in the membrane potentials in response to various taste stimuli and odorants were examined using taste organs, neuroblastoma, isolated olfactory cells and liposomes. Exchanges of ions in external medium with impermeable ions did not affect the changes of the membrane potentials in response to chemical stimuli. The membrane potential changes of lipid bilayer membranes in response to bitter substances and odorants occurred under the conditions that ion concentration at both sides of membranes is the same, suggesting that the membrane potential changes are induced by changes in the phase boundary potentials.

Report

(1 results)
  • 1986 Final Research Report Summary

Research Products

(22 results)

All Other

All Publications (22 results)

  • [Publications] Kumazawa,T.;Kashiwayanagi,M.;Kurihara,K.: Brain Research. 333. 27-33 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Ohno,T.;Yoshii,K.;Kurihara,K.: Comp.Biochem.Physiol.82A. 153-159 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Kashiwayanagi,K.;Kurihara,K.: Brain Research. 359. 97-103 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Kumazawa,T.;Kashiwayanagi,M.;Kurihara,K.: Biochimica et Biophysica Acta. 888. 62-69 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Yoshii,K.;Yokouchi,C.;Kurihara,K.: Brain Research. 367. 45-51 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Kurihara,K.;Yoshii,K.;Kashiwayanagi,M.: Comp.Biochem.Physiol:. 85A. 1-22 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Yoshii,K,;Kiyomoto,Y.;Kurihara,K.: Comp.Biochem.Physiol.85A. 501-507 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Kashiwayanagi,M.;Sai,K.;Kurihara,K.: J.Gen.Physiol.(1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Nomura,T.;Kurihara K.: Biochemistry. (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Kurihara,K.;Nomura,T.;Kashiwayanagi,M.;Kumazawa,T.: Transducres´87. (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Nomura,T.;Kurihara,K.: Biochemistry. (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Kumazawa, T.; Kashiwayanagi, M.; Kurihara, K.: "Neuroblastoma cell as a model for a tast cell: Mechanism of depolarization in response to various bitter substances." Brain Research. 333. 27-33 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Ohno, T.; Yoshii, K.; Kurihara, K.: "Quantitative analysis on discrimination of various odorants at receptor sites of the frog olfactory cell revealed by a cross adaptation method." Comp. Biochem. Physiol.82A. 153-159 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Kashiwayanagi, K.; Kurihara, K.: "Evidence for non-receptor odor discrimination using neuroblastoma cells as a model for olfactory cells." Brain Research. 359. 97-103 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Kumazawa, T.; Kashiwayanagi, M.; Kurihara, K.: "Contribution of electrostatic and hydrophobic interactions of bitter substances with taste receptor membranes to generation of receptor potentials." Biochimica et Biophysica Acta. 888. 62-69 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Yoshii, K.; Yokouchi, C.; Kurihara, K.: "Synergistic effects of 5'-nucleotides on rat taste responses to various amino acids." Brain Research. 367. 45-51 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Kurihara, K.; Yoshii, K.; Kashiwayanagi, M.: "Transduction mechanisms in chemoreception (Review)" Comp. Biochem. Physiol.85A. 1-22 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Yoshii, K.; Kiyomoto, Y.; Kurihara, K.: "Taste receptor mechanism of salts in frog and rat." Comp. Biochem. Physiol.85A. 501-507 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Kashiwayanagi, M.; Sai, K.; Kurihara, K.: "Cell suspensions from porcine olfactory mucosa; Changes in membrane potential and membrane fluidity in response to various odorants." J. Gen. Physiol. in press. (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Nomura, T.; Kurihara, K.: "Effects of changed lipid composition on responses of liposomes to various odorants: A possible mechanism of odor discrimination." Submitted to Biochemistry. (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Kurihara, K.; Nomura, T.; Kashiwayanagi, M.; Kumazawa, T.: "Transduction mechanism of chemical sensors in biological sytems and its application to artificial membrane sensors." Transducers'87. (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Nomura, T.; Kurihara, K.: "Liposomes as model for olfactory cells: Changes membranes potential in response to various odorants." Submitted to Biochemistry. (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary

URL: 

Published: 1987-03-30   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi