| Project/Area Number |
60480485
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Laboratory animal science
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| Research Institution | NAGASAKI UNIVERSITY |
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Principal Investigator |
SATO HIROSHI NAGASAKI UNIVERSITY SCHOOL OF MEDICINE, Assistant Professor, 医学部, 助教授 (50072947)
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| Co-Investigator(Kenkyū-buntansha) |
MIYATA HIRONORI NAGASAKI UNIVERSITY SCHOOL OF MEDICINE, Assistant, 医学部, 助手 (70174191)
YAMANOUCHI KAZUYA INSTITUTE FOR MEDICAL SCIENCE, TOKYO UNIVERSITY, Professor, 医科学研究所, 教授 (30072888)
HINO SHIGEO NAGASAKI UNIVERSITY SCHOOL OF MEDICINE, Assistant Professor, 医学部, 助教授 (70012763)
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| Project Period (FY) |
1985 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥5,100,000 (Direct Cost: ¥5,100,000)
Fiscal Year 1986: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1985: ¥3,500,000 (Direct Cost: ¥3,500,000)
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| Keywords | Lymphocytic choriomeningitis virus / LCM virus / Rodents / 間接蛍光抗体 |
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| Research Abstract |
INDIRECT FLUORESCENT ANTIBODY METHOD WAS APPLIED FOR A DETECTION OF LYMPHOCYTIC CHORIOMENINGITIS VIRUS(LCMV) ANTIBODY IN COLONIES OF LABORATOREY ANIMALS IN JAPAN. THE RESULTS SHOWED THAT THE ANTIBODY EXIST IN SPF MICE(3/152, 2.2%) AND CONVENTIONAL MICE(30/539, 5.6%) WITH LOW TITERS RANGING FROM 1:10 TO 1:160. THE ANTIBODY WAS ALSO DETECTED IN 2.2%(2/89) OF SYRIAN HAMSTERS, AND 2.9%(2/68) OF APODEMUS AGRARIUS, 21.4%(3/14) OF JAPANESE HARVEST MICE WHICH HAVE BEEN MAINTAINED AS LABORATORY COLONY FOR SEVERAL YEARS. FURTHERMORE, 8.6%(19/222) ANTIBODY POSITIVE ANIMALS WITH COMPARATIVE TITERS WERE DETECTED IN THE SEVERAL SPECIES OF RODENTS CAPTURED IN WESTERN PARTS OF JAPAN. HOWEVER, THE ANTIBODY WAS NOT DEMONSTRATED IN THE SERA OF MONGOLIAN GERBIL, SUNCUS MURINUS, GUINEA PIG AND RAT, THUS FAR. THESE RESULTS SUGGEST THAT LCMV INFECTION IS PRESENT IN LABORATORY ANIMALS AND WILD RODENTS IN JAPAN AS WELL AS WESTERN COUNTRIES, AND POINTED OUT THE IMPORTANCE OF MICROBIOLOGICAL MONITORING FOR LCMV IN LABORATORY AND WILD RODENTS. A TRIAL OF VIRUS ISOLATION WAS RESULTED IN NEGATIVE. REISOLATION STUDY OF LCMV FROM WILD RODENTS SHOULD BE CONDUCTED IN NEAR FUTURE.
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