• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Induction of in vivo somatic mutations by low dose radiation in mice -threshold and dose rate effects-.

Research Project

Project/Area Number 60480505
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 放射線5生物学
Research InstitutionOsaka University

Principal Investigator

NOMURA Taisei  Osaka University,Faculty of Medicine, 医学部, 教授 (90089871)

Project Period (FY) 1985 – 1987
Project Status Completed (Fiscal Year 1987)
Budget Amount *help
¥6,900,000 (Direct Cost: ¥6,900,000)
Fiscal Year 1987: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1986: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1985: ¥3,500,000 (Direct Cost: ¥3,500,000)
KeywordsLow dose radiation / Low dose rate radiation / in vivo somatic mutation / Mouse / 遺伝子突然変異 / 閾値 / 線量率効果 / ヒト遺伝リスク / マウス体細胞突然変異 / X線 / 毛色劣性遺伝子PT-HTF1法 / 精原細胞
Research Abstract

Genetic effects of low dose radiation were examined with the use of in vivo somatic mutation method (PH-HTF1)in mice.Dose rate effects were also tested with the same method.
1.Induction of in vivo somatic mutations with 10R of X rays:PT female was mated with HT males, and then 10R of X rays(55-62R/min)was given on the 10-1/2 the day of gestation.F1 mice (a/a,b/+,pc^<ch>/++,d/+,pa/+,pe/+,in/+)were sacrificed 6 weeks after birth,and colored spots were examined on the non-agouti(black)background.30 spots were found in 746F1 offspring(0.04)after 10R exposure,whereas 22spots were found in 750 unexposed controls. There is no significant diviation from linear dose response relationship between 0 and 130 R.
2.Induction of in vivo somatic mutations by chronic exposure:110 R of X rays were given at different dose rate,55-62R/min,1 R/min,0.5R/min in the same time schedule.32.5R was also given at 0.1R/min.Numbers of mutants per F1 were 26/218(0.119),23/116(0.198),25/161 (0.155),and 8/59(0.136),respectively.
3.Numbers of survived target cells and mutation rate per locus per R:Numbers of target cells were estimated from the ratio of total numbers of hair and mutant hairs.Estimated numbers of target cells were 530,1211,1853,and 3500,respectively.Consequently,mutation rate per locus per R was 1.9x10^<-7>,1.6x10^<-7>,0.8x10^<-m>,and 1.1x10^<-7>at dose rate of 55-62 R/min 1.0R/min,0.5R/min,and 0.1R/min,respectively,The results were compatible with those by specific locus mutations for spermatogonia.
Threshold/Dose rate effect/Human genetic risk

Report

(2 results)
  • 1987 Final Research Report Summary
  • 1986 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] Nomura, T.: Mutation Research. 190. 25-29 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Nomura, T.: Mutation Research.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Gotoh, H.: Mutation Research.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 野村大成: 病態生理. 35. 769-773 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] 野村大成: 蛋白質, 核酸, 酵素. 32. 292-298 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Nomura, T.: "Genetic Toxicology of Enveronmental Chemecale (Part B)" Alan R. Liss, 569 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Nomura,T.: "Killing of preimplantaion embryos by main ingredients of cleanesers AS and LAS" Mutation Research. 190. 25-29 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Nomura,T.: "X-ray and chemically induced germ-line mutation causing phenotypical anomalies in mice." Mutation Research. (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Gotoh,H.: "Inhibiting effects of nicotinamide on urethane-induced malformations and tumors in mice." Mutation Research. (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Nomura,T.: "Heritable alterations causing cancer." Medicina Philosophica. 5. 769-773 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Nomura,T.: "Heritable factors in carcinogenesis-cancer-causing mutations." Protein,Nucleic Acid and Enzyme. 32. 292-298 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Nomura,T.: Alan R.Liss. Genetic Toxicology of Environmental Chemicals(PartB)., 569(8) (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Nomura,T.: Genetic Toxicology of Environmental Chemicals. PartB. 13-20 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Nomura,T.: Mutation Research. 190. 25-29 (1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] Nomura,T.: Functional Effects on the Offspring after Parental Drug Exposure. (1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] 野村大成: 病態生理. 5. 769-773 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] 野村大成: 蛋白質.核酸.酸素. (1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] Nomura,T.: Mutation Research. (1987)

    • Related Report
      1986 Annual Research Report

URL: 

Published: 1987-03-31   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi