| Project/Area Number |
60480511
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
分子遺伝学・分子生理学
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| Research Institution | University of Tsukuba (1986-1987)
Kyoto University (1985) |
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Principal Investigator |
AIBA Hiroji Department of Chemistry, University of TSukuba, 化学系, 助教授 (20025662)
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| Project Period (FY) |
1985 – 1987
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| Project Status |
Completed (Fiscal Year 1987)
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| Budget Amount *help |
¥6,700,000 (Direct Cost: ¥6,700,000)
Fiscal Year 1987: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1986: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1985: ¥4,900,000 (Direct Cost: ¥4,900,000)
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| Keywords | cAMP / cAMp receptor protein / promoter / regulation of transcription / CRPレギュロン / プロモーター |
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| Research Abstract |
The transcription of Escherichia coli cya gene by cAMP and its receptor protein(CRP) has been studied. The transcription of cya gene was shown to be negatively regulated by cAMP-CRP in vitro. The cAMP-CRP complex binds specifically to the cya promoter region to inhibit the transcription. The negative regulation of the cya transcription was also shown in vivo by S1 nuclease and RNA dot blot assays. The cya-lac gene fusion studies gave another evidence for the cya regulation by cAMP- CRP.
The whole genome of E.coli was digested by a restriction enzyme and the resulting DNA fragments were randomly cloned into a promoter selection cloning vector. Among about 100 clones, 5 have been shown to contain promoters that can be activated by cAMP. Additionally one promoter, which transcription is represses by cAMP, was also isolated. The DNA sequences of these promoters have been determined. Most of promoters sequenced turned out to corresponds to unknown genes.
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