Amino sugars and their related compounds having nucleic acid- scissoring activity.
| Project/Area Number |
60490024
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
広領域
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| Research Institution | Fukuoka University |
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Principal Investigator |
WATANABE kenji Fukuoka Univ., Foc.Pharm.Sci., Professor, 薬学部, 教授 (40078689)
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| Co-Investigator(Kenkyū-buntansha) |
KAKITA Yukari Fukuoka Univ., Fac,Pharm.Sci., Assistant, 薬学部, 副手 (80194679)
NAKASHIMA Yukihiko Fukuoka Univ., Foc.Pharm.Sci., Assistant., 薬学部, 助手 (50164174)
SUMOTO Kunihiro Fukuoka Univ., Fac.Pharm.Sci., Assistant Professor, 薬学部, 助教授 (00078677)
YAMAGUCHI Tadatoshi Miyazaki Med.Col., Dept.Hyg., Instructor, 講師 (80037598)
MIHASHI Kunihide Fukuoka Univ., Fac.Pharm.Sci., Assistant Professor, 薬学部, 助教授 (60078681)
林田 みき枝 福岡大学, 薬学部, 助手 (50173050)
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| Project Period (FY) |
1985 – 1987
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| Project Status |
Completed (Fiscal Year 1987)
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| Budget Amount *help |
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1987: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1986: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | Aminosugars / D-glucosamine / DNA damage / DNA切断 / 活性酸素 / マキサム・ギルバート法 / pBR322 / プラスミド / DNA / DNA切断反応 / Dーグルコサミン / 3ーアミノー3ーデオキシアロース |
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| Research Abstract |
DNA strand scisson, change of ccc-DNA into oc- and linear-DNA and urther fragmented DNA, by various aminosugars was investigated through agarose gel electrophoresis.
D-Glucosamine, D-mannosamine, D-galactosamine and D-isoglucosamine had the DNA strand-scissoring activity. Both the C-2 amino group and C-1 aldehyde one of the molecules were indespensable for their activities. D-Glucosamine, auto- claved at 120゜C in agueous solution, had a greater activity than D-glucosamine itself. The reaction was stimulated by Cu^<2+> and inhibited by catalase, SOD and some radical scavengers. Some free radicals, particularly hydroxyl radical, generated during autoxidation of aminosugrs in agueous solution, were thought to be involved in the DNA strand strand scission. D-Glucosamine induced alkaline- be involved in the DNA strand strand scission. D-Glucosamine induced alkaline- lable damage preferentially at pyrimidine residues in the dinucleotide sequence of pyrimidine-purine (5'-> 3').
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Report
(2 results)
Research Products
(5 results)
