| Project/Area Number |
60540438
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
植物生理学
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| Research Institution | Kagoshima University |
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Principal Investigator |
HIGASHI Shiro Fac. Science, Kagoshima Univ., Professor, 理学部, 教授 (60041216)
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| Co-Investigator(Kenkyū-buntansha) |
ABE Mikiko Fac. Science, Kagoshima Univ., Asistant Professor, 理学部, 助教授 (00107856)
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| Project Period (FY) |
1985 – 1987
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| Project Status |
Completed (Fiscal Year 1987)
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| Budget Amount *help |
¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1987: ¥200,000 (Direct Cost: ¥200,000)
Fiscal Year 1986: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1985: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | Legume-root nodule / Leghemoglobin / Lb-synthesized cell / Lb-induction gene(Lb-ind gene) Lb-cDNA / Lb-cDNA / Lb-ind gene / transfarmants / 根粒菌 / 感染機構 / Lb-発現機構 |
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| Research Abstract |
Leghemoglobins (Lbs) are small monomeric heme proteins synthesized exclusively in the root nodules that develope due to the rhizobial infection to leguminous plant. The induction of Lb-genes occurs only after bacterial infection but is independent of the expression of nif-gene in bacteroid. The Lb-gene expression of root cells are induced by some signals derived from the invaded bacteria. The signals are not Known about the details although nod- and nif-gene have been analyzed about the loci on Sym-plasmid and nucleotide sequences.
The cell, leghemoglobin is synthesized at the most early step of nodulation, has been determined by an indirect-immunological technique.
For analysis the expression of Lb-induction gene(Lb-ind gene), Lb-gene has been cloned with the mRNA of root nodule : reverse transcriptase system. The total DNA from R. trifolii 4S(Nod^+, Lb^+, Fix^+) was isolated and digested with Sau3AI. The DNA fragments were ligated with cosmid vector pCKS 13 and packaged in <lambda>-phage head. The recombinant DNA including in E. coli cells were transferred to R. trifolii Qn1(Nod^+,Lb^-, Fix^-) by triparental mating method, and then inoculated to seedilings of white clover. A strain NL18(Nod^+, Lb^+, Fix^-) was screened out form a nodule. The locus and size of Lb-ind gene on the cloning DNA from R. trifolii 4S was discussed in this study.
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