| Project/Area Number |
60560095
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
応用生物化学・栄養化学
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| Research Institution | Tottori University |
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Principal Investigator |
MORISHIMA Isao Tottori University, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (30032296)
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| Co-Investigator(Kenkyū-buntansha) |
UENO Teruo Tottori University, Faculty of Agriculture, Professor, 農学部, 教授 (50151816)
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| Project Period (FY) |
1985 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1986: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1985: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | Antibacterial protein / Silkworm / Bombytoxin / Bombyx mor: / 家蚕 |
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| Research Abstract |
Injection of live or heat-killed bacteria into larvae of the silkworm, Bombyx mori induced antibacterial activities in the hemolymph. Although there was no specificity of injected bacteria for the induction of antibacterial activity, yeast cells, fungus spores and saline alone were not effective as inducing agent.
The antibacterial activities were separated into at least six bands on native poly-acrylamide gel electrophoresis, which were sensitive to trypsin. The antibacterial activities were separated from lysozyme activity on CM-cellulose column chromatography. Two of these proteins (AP-A and AP-B) were purifies to apparent homogenity. They were heat-stable and showed characteristic properties of cecropins. The proteins repressed growth of various bacteria at a concentration of 0.1 ug/ml.
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