| Project/Area Number |
60560131
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
製造化学・食品
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| Research Institution | THE UNIVERSITY OF TOKYO |
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Principal Investigator |
YOKOTA TAKAO The University of Tokyo, DEPT OF AGRICULTURE, 農学部, 助手 (40011986)
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| Project Period (FY) |
1985 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1986: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1985: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | Brassinosteroid / Brassinolide / Castasterone / Biosynthesis / 代謝 |
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| Research Abstract |
Tritium-labelled brassinolide and castasterone were prepared by reduction of dolicholide and dolichosterone respectively. Radioactive reduction products were purified by thin-layer and high performanceliquid chromatography, affording radio-labelled brassinolide and castasterone: specipic radioactivity of them was about 4Ci/mM.
3-leaf-stage rice seedlings were fed with ( <^3H> )castasterone for 6 hr through the roots. Portion of the treated seedlings were further incubated for 10 days in castasterone-free medium. These plant materials were analysed for the metabolites of castasterone. Thus, it was found that castasterone is absorbed by roots and transported to shoots. It was also found that castasterone was found to be rapidly converted into water-soluble metabolites. The structures of them seemed to be acidic conjugates rather than glycosides on the basis of chromatographic analysis and hydrolysis. Ten days after the treatment, most of castasterone absorbed was changed into polar metabolites, while unchanged castasterone accounted for only 0.43%. Castasterone, a hypothetical precursor of brassinolide was never changed into brassinolide. Fate of ( <^3H> )brassinlode was similarly pursued and the result was analogous to that of castasterone.
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