Production of Interspecific Chimeric Blastocysts.

Research Project

Project/Area Number	60560271
Research Category	Grant-in-Aid for General Scientific Research (C)
Allocation Type	Single-year Grants
Research Field	畜産学(含草地学)
Research Institution	Iwate University
Principal Investigator	TAKAHASHI Jutaro Derartment of Agriculture, Iwate University, Associate Professor, 農学部, 助教授 (00004929)
Project Period (FY)	1985 – 1986
Project Status	Completed (Fiscal Year 1986)
Budget Amount *help	¥1,900,000 (Direct Cost: ¥1,900,000) Fiscal Year 1986: ¥500,000 (Direct Cost: ¥500,000) Fiscal Year 1985: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywords	Interspecific chimeras / Development of embryos / Culture of embryo in vitro / キメラ胚盤胞
Research Abstract	The production of interspecific chimeric embryos may be available for analysis of the " capacity " of embryos in early developmental stages. As a culture medium, Dulbecco's modified eagle medium with 0.3 % bovine serum albumin ( pH 7.4 ) was used for the production of xenogeneic chimeric embryos ( Mouse:Rat, Mouse:Hamster, Rat:Hamster ). From a total of 53 aggregates of a mouse embryo with a rat embryo, 43 ( 81.1% ) developed into chimeric blastocysts, and this rate was similar to those of the Mouse:Mouse and the Rat--Rat systems. In the Hamster:Hamster and the Rat:Hamster systems, no chimeric blastocyst was obtained. However, in combination of mouse and hamster embryos, only 3 of 33 aggregates developed into chimeric blastocysts. These results may indicate that there is a great discrepancy between families in developmental biology.