| Project/Area Number |
60570019
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Tokyo Women's Medical College |
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Principal Investigator |
TSUYUKA KUSHIDA Tokyo Women's Medical College, Professor of Anatomy, 第一解剖学教室, 教授 (90055723)
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| Co-Investigator(Kenkyū-buntansha) |
HARUYUKI IIJIMA Tokyo Women's Medical College, Professor of Anatomy, 第一解剖学教室, 助手 (40130231)
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| Project Period (FY) |
1985 – 1987
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| Project Status |
Completed (Fiscal Year 1987)
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| Budget Amount *help |
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1987: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1986: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1985: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | Semi-thin sections / Plastic embedding / Mouse testis / 三次元構造 / 精巣組織 |
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| Research Abstract |
The usual semi-this sections is applicate to testis. A modification for water miscible methacrylate embedded tissue suitable for correlative light and electron microscopic studies, which makes it possible to semi-thin testis tissue section, is described. An improved eopxy resin embedding method has been debised for steroscopic observations under 200kV and a tilt angle of <plus-minus>10゜ using a transmission electron microscope with a side entry gonimeteサク
The newborn, juvenile and adult mice were used in this study. Small piece of tissue specimens were fixed in a mixture of glutaraldehyde-formaldehyde or 2%osmium tetrozide and 2.5%glutaraldehyde. They were embedded HPMA-quetol 523-MMA or Quetol 812NSA mixture. Semi-thin sections approximately 0.5-1.0um thich were cut with glass knives on an ultramicrotome, mounted on grid were stained with H-E and PAS. For electron microscopy, en bloc-stained tissue block were embedded in the resin mixture. Identical sites on such sections approximately 0.5-1.0um thich colud be distinctly examined with 200kV at low using light and electron microsocope. Thus, photographs and electron micrographs of identical sites on testis tissue could be compared exactly.
In the present study, the intracelluar structures of spetmatogenic cells on a spermatogenesis, Sertoli cells ans Leyding cells are clearly seen compared with usual ultra-thin sections. For instance, the three-dimensional organization of mid-piece-of spermatozoa can be observed distinctly.
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