Studies on the mechanism of cadmium-induced toxic effects(Autoradiographic demonstration of cadmium unbound to metallothionein)

Research Project

Project/Area Number	60570020
Research Category	Grant-in-Aid for General Scientific Research (C)
Allocation Type	Single-year Grants
Research Field	General anatomy (including Histology/Embryology)
Research Institution	St. Marianna University School of Medicine
Principal Investigator	SUZUKI Takuro St. Mariannna University School of Medicine, 医学部, 教授 (00057465)
Co-Investigator(Kenkyū-buntansha)	SUZUKI Kazuo T. National Institute for Environmental Study, 環境保健部, 室長 (90109918) SUMI Yawara St. Marianna University School of Medicine, 医学部, 講師 (20121197)
Project Period (FY)	1985 – 1986
Project Status	Completed (Fiscal Year 1986)
Budget Amount *help	¥1,600,000 (Direct Cost: ¥1,600,000) Fiscal Year 1986: ¥400,000 (Direct Cost: ¥400,000) Fiscal Year 1985: ¥1,200,000 (Direct Cost: ¥1,200,000)
Keywords	autoradiography / cadmium / thiazolylazo-naphthol
Research Abstract	A new autoradiographic technique for demonstrating trace amounts of "free cadmium" which remains unbound to metallothionein in a tissue section is described. This was realized by the use of [14C-1,4,5,8-naphthol]-1-(thiazolylazo)-2-naphthol (TAN) instead of a metal-radioisotope. In this method, tissue sections were stained with an aqueous solution of labeled TAN, and then the sections were placed in 2-propanol in order to remove any unreacted TAN. The decontaminated sections were finally subjected to the usual autoradiographic procedure. A high concentration of grains overlying coloured regions was observed in sections prepared from tissues injected with a diluted aqueous solution of cadmium acetate. This demonstrated the feasibility of the differential demonstration of cadmium according to its chemical forms in tissues. This technique may yield additional information as to the toxicity of cadmium in biological systems.