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Screening of Visual Center Specific Marker Molecules by Monoclonal Antibody Technique

Research Project

Project/Area Number 60570029
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 神経解剖学
Research InstitutionKyoto Prefectural University of Medicine

Principal Investigator

FUJISAWA Hajime  Associate Professor, 医学部, 助教授 (60079689)

Co-Investigator(Kenkyū-buntansha) TAKAGI Shin  Research Associater, 医学部, 助手 (90171420)
Project Period (FY) 1985 – 1986
Project Status Completed (Fiscal Year 1986)
Budget Amount *help
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1986: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1985: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsXenopus frog / Visual System / neuronal recognition molecules / monoclonal antibody / immunohistochemistry / 眼球移植 / 遺伝子クローニング
Research Abstract

To clarify the molecular background of the specific neuronal connection between retinal axons and visual centers, we undertook to screen target specific maker molecules by the monoclonal antibody producing technique. A monoclonal antibody A5 (MAb-A5) which was prepared against mechanically dissociated tectal cells of Xenopus tadpoles (at stages 51-53) bound to laminas 8 and 9 (the sites of optic nerve innervation) of the tectum and also other minor visual centers in the diencephalon and midbrain, but not to the retina, optic nerve or optic tract. The epitope of the MAb-A5 (A5 antigen) was a cell surface protein molecule approximately 140 K in molecular weight. The A5 antigen appeared in the tectum at first at stage 39 of development when tectal invasion by retinal axons was initiated. Besides the visual centers, the A5 antigen also appeared in very restricted regions in the medulla and spinal cord. When eyes were transplanted on the back of Xenopus embryos, retinal axons from the transplanted eye preferentially arrived at the A5 antigen-positive regions in the medulla and spinal cord. These findings suggest that the A5 antigen may be a marker molecule of optic nerve targets, and may play important roles in the preferential guiding or trapping of ingrowing retinal axons.

Report

(1 results)
  • 1986 Final Research Report Summary
  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Hajime Fujisawa: J.Comparative Neurology. (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Shin Takagi: Developmental Biology. (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Hajime Fujisawa: Progress in Brain Research. 71. 75-88 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Hajime Fujisawa: Development,Growth & Differentiation. 29. 105-112 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Hajime Fujisawa: Progress in Developmental Biology. Part B. 109-112 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Hajime Fujisawa: "Mode of growth of retinal axons within the tectum of Xenopus tadpoles, and implications in the ordered neuronal connection between the retina and the tectum" J. Comparative Neurology. (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Shin Takagi: "Specific cell surface labels in the visual centers of Xenopus laevis tadpole identified using monoclonal antibodies" Developmental Biology. (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Hajime Fujisawa: "A5 antigen: Putative involvement in neuronal recognition in amphibian retinal central projections"

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Hajime Fujisawa: "Axonal growth in developing and regenerating amphibian retinotectal projection" Progress in Brain Research. 71. 75-88 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Hajime Fujisawa: "How do retinal axons arrive at their targets?: cellular and molecular approaches" Development, Growth & Differentiation. 29. 105-112 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary

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Published: 1987-03-31   Modified: 2016-04-21  

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