| Project/Area Number |
60570040
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
General physiology
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| Research Institution | Nagoya City University Medical School |
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Principal Investigator |
HOTTA Ken Nagoya City University Medical school, 医学部, 教授 (90079971)
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| Co-Investigator(Kenkyū-buntansha) |
TAKAGI Takuji Nagoya City University Medical school, 医学部, 助教授 (70080075)
YAMAMOTO Yoshimichi Nagoya City University Medical school, 医学部, 助手 (80145755)
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| Project Period (FY) |
1985 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1986: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1985: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | Smooth muscle / Single cell / Ca transient / Ca store / Quin2 / フィラ2 |
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| Research Abstract |
We have shown previously(J.J.P.35:1079,1985) that, intracellular free Ca in smooth muscle cells(guina pig jejunum) increased transiently upon stimulation followed by the mechanical response. We explored this study to the single cell using Fura 2.
The isolated cell was obtained from taenia coli of guiea pig according to the method described by Momose & Gomi in 1979(J.Pharmaco dynamics 1:184). Fura2 was loaded into the cell by incubating the sample in HEPES buffer containing Fura 2AM for 30 min at 37゜C. The photomultiplier was set to recieve the emission(470nm) light from only one cell on the stage of microscope.
Upon application of electric pulse, intensity of fluorecence light from the muscle begun to increase immedately after application of stimulus reaching to the maximum within one sec and returned to the original level after 2 sec. Concentration of intracellular freeCa was estimated as approximately <10^(-7)> M and the order of <10^(-6)> M,at rest and the maximum,respectively. The rate of light intensity change was faster than that observed previously in the tissue preparation. The speed of contraction following the stimulation was much slower than that of intracellular Ca transient. The results obtained here,together with previous one,suggest that intracellulary stored Ca may play certain role for the mechanical activation of the smooth muscle cell.
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