| Project/Area Number |
60570073
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Neurophysiology and muscle physiology
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| Research Institution | Okazaki National Research Institutes |
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Principal Investigator |
SUGIYAMA Hiroyuki National Institute for Physiological Sciences, Associate Professor, その他, 助教授 (20124224)
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| Co-Investigator(Kenkyū-buntansha) |
ITO Isao Shimane Medical University, Research Assistant, 日本学術振興会特別研 (20183741)
HIRONO Chikara National Institute for Physiological Sciences, Research Associate, 生理学研究所, 協力研究員 (10199135)
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| Project Period (FY) |
1985 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1986: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1985: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | Messenger RNA / Xenopus laevis / Neurotensin / Acetylcholine receptor / Inositol phospholipid / Inositol trisphosphate / G-protein / アフリカツメガエル |
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| Research Abstract |
The intracellular reaction mechanism underlying electrophysiological responses evoked by neurotensin was studied using Xenopus laevis oocytes injected with mRNA isolated from rat brains.
A few days after the injection of mRNA, oocytes were found to acquire sensitivity to neurotensin and substance P, as well as to ACh (muscarinic).
From detailed analyses of responses evoked by these stimulants, it is concluded that neurotensin responses and muscarinic ACh responses of Xenopus oocytes induced by rat brain mRNA may most likely share a common reaction mechanism. The reaction sequence includes the activation of receptors, activation of inhibitory guanin nucleotide-binding regulatory protein, production of inositol 1,4,5-trisphosphate, intracellular <Ca^(2+)> mobilization, and increased membrane permeability to <Cl^-> ions.
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