| Project/Area Number |
60570108
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
General medical chemistry
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| Research Institution | Niigata University |
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Principal Investigator |
ARAKI Shigeko Brain Research Institute Niigata Univ. Assistant, 脳研究所, 助手 (70018604)
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| Co-Investigator(Kenkyū-buntansha) |
ABE Sachiko Brain Research Institute Niigata Univ. Assistant, 脳研究所, 助手 (60018603)
SATAKE Mei Brain Research Institute Niigata Univ. Professor, 脳研究所, 教授 (70018589)
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| Project Period (FY) |
1985 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1986: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1985: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | phosphonoglycosphingolipids / Aplysia nervous tissue / chemical structure / tissue location / immunohistochemistry / gangliosides |
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| Research Abstract |
On two-dimensional TLC, each tissue of Aplysia showed a pattern of phosphonoglycosphingolipids characteristic to the tissue. FGL-IIa and FGL-IIb were specific to the nerve fiber and FGL-V (G-13) was seemed to be specifically concentrated in the nervous tissues. Three novel phosphonoglycosphingolipids (FLG-IIa, FGL-IIb and FGL-V) were isolated from the nervous tissues of Aplysia kurodai, using three systems of silicic acid column chromatography and characterized. FGL-IIa was monophosphonoglycosphingolipid having an oligosaccharide chain consisting of glucose, galactose and galactosamine. FGL-IIb was also monophosphonoglycosphingolipid having fucose, glucose, galactose and galactosamine as an oligosaccharide moiety. FGL-V (G-13) seemed to be diphosphonoglycosphingolipid having an oligosaccharide chain consisting of glucose, galactose, galactosamine and 4-0-methylglucosamine.
Antisera against FGL-IIa and FGL-IIb mixture were raised in rabbits. Two-dimensional thin-layer chromatography combined with immunoautoradiography using the antisera and <^(125)I> -Ig,F(ab')2, revealed nine immunoreactive glycolipids in extracts from the nerve fiber. By immunohistochemistry with the antisera and HRP-labelled antirabbit IgG, distinct and strong staining was observed in the nerovus tissue. Nerve fibers in the skin and muscle tissue were also stained.
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