• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Histopathological study of collagen components in pulmonary fibrosis

Research Project

Project/Area Number 60570140
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Human pathology
Research InstitutionTOKYO MEDICAL AND DENTAL UNIVERSITY

Principal Investigator

MATSUBARA. Osamu  TOKYO MEDICAL AND DENTAL UNIVERSITY, Assistant Professor, 医学部, 助教授 (40107248)

Project Period (FY) 1985 – 1986
Project Status Completed (Fiscal Year 1986)
Budget Amount *help
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1986: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1985: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsPulmonary fibrosis / collagen / Fibrosis / パラコート
Research Abstract

For analysis of collagen components in pulmonary fibrosis, solubilization of collagen components in lungs was attempted. For rat lungs papain was effective with complete solubilization, but for human lungs solubilization by pepsin left 27% of the total dry weight of the initial materials, while papain was better in solubilization of fibrotic lungs (residues about 13%). Collagen solubilized was analysed by SDS-urea polyacrylamide gel electrophoresis. The relative amout of type <III> to type <I> collagen in rat lungs increased a few days after injection of paraquat, then decreased to the normal level. The relative ratios of alphaI( <III> ) to alphaI( <I> ) and of alphaI( <I> ) to alphaI( <V> ) of solubilized collagen from human lungs were enhanced in the human fibrotic lungs. The amount of collagen solubilized and recovered was less in normal lungs, one-fourth of that in fibrosis.
The resultscan be interpreted in three ways:
1) Normal lungs contain a higher amount of proteases-labile collagens which may be digested away during the protease treatment;
2) Normal lungs contain a higher potential activity for collagen degradation which may be activated during the protease treatment;
3) Normal lung tissues contain a higher amount of protease-insoluble collagen.
Changes in the architectures of normal and fibrotic lungs were examined immunohistologically with specific antibodies against types <I> , <III> and <IV> collagens. The relation between biochemical analysis and immunohistological observations must be further examined.

Report

(1 results)
  • 1986 Final Research Report Summary
  • Research Products

    (2 results)

All Other

All Publications (2 results)

  • [Publications] Vircnows Archiv A.408. (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Virchows Archiv A.408. (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary

URL: 

Published: 1987-03-31   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi