| Project/Area Number |
60570149
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Human pathology
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| Research Institution | Fukouka University |
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Principal Investigator |
IWASAKI Hiroshi Fukuoka University Associate Professor, 医学部, 助教授 (90101170)
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| Project Period (FY) |
1985 – 1987
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| Project Status |
Completed (Fiscal Year 1987)
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| Budget Amount *help |
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1987: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1986: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1985: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | soft tissue / histiocyte / fibroblast / myofibroblast / macrophage / monocyte / reticuloendothelial system / 悪性線維性組織球腫 / モノクローナル抗体 / 細胞膜抗原 / 細胞培養 |
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| Research Abstract |
Using whole cell antigens prepared from the established lines of human malignant fibrous histiocytoma(MFH),we have generated four different monoclonal antibodies (FU3,FU4,FU5,FU6) by a mouse hybridoma technique.By indirect immunoperoxidase in frozen tissue sections,FU3 and FU4 revealed strong staining of perivascular mesenchymal cells and fibroblasts.Macrophages in granulation tissues as well as monocytes and other blood cells in normal peripheral blood were uniformly negative for the antigen detected by FU3.Immunoelectron microscopic studies demonstrated positive reactions with FU3 and FU4 on the surface of MFH cell membrane,suggesting that these antibodies recognized cell surface antigens.MFH shares antigenicity with perivascular mesenchymal cell and fibroblast.MFH may have a common origin from the perivascular mesenchymal cells that are supposed to have a potential for multidirectional differentiation.
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