Tissue Culture Studies On Regulatory Factors For Non-neoplastic Schwann Cell Proliferation.
| Project/Area Number |
60570151
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Experimental pathology
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| Research Institution | Asahikawa Medical College |
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Principal Investigator |
SHIMODA Akihisa The lst Department of Pathology, Asahikawa Medical College, 医学部, 教授 (60041810)
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| Co-Investigator(Kenkyū-buntansha) |
MURAOKA Shunji The 1st Department of Pathology, Asahikawa Medical College, 病理学第一講座, 講師 (60142816)
TAKAHASHI Tatsuro The 1st Department of Pathology, Asahikawa Medical College, 病理学第一講座, 講師 (60041844)
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| Project Period (FY) |
1985 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1986: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1985: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | Schwann cells / Spinal sensory ganglia / Tissue culture / Tellurium neuropathy / 細胞増殖 |
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| Research Abstract |
This studies were undertaken to evaluate whether the culture media of sciatic nerve in the process of Schwann cell proliferation in vivo also have the same effect on Schwann cells in vitro which are beforehand prepared from spinal sensory ganglia. Four steps of experiments designed are as follows.
Step 1: Preparation and maintenance of Schwann cell in vitro. Step 2: Preparation of tellurium neuropathy(segmental demyelination) in sciatic nerve as in vivo model of Schwann cell proliferation. Step 3: Culture and collection of culturing media of sciatic nerves from two sources which are the rats suffered from tellurium neuropathy (T) and healthy rats (C) as control. Step 4: Effects of collected culturing media on Schwann cells prepared by Step 1.
For Step 1 experiment, spinal sensory ganglia of 19 day rat fetuses were cultured according to the method by Wood (1976), and relatively pure population of Schwann cells are prepared. In Step 2, weanling rats fed diet containing 1.5 % tellurium for
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4 to 7 days revealed Schwann cell proliferation in affected sciatic nerves (T) without any changes by control diet (C). Therefore, sciatic nerves from 5 days on each diet rats were cultured for Step 3. On 1,3,5 and 7 days in vitro, culturing media was collected prior to re-feeding, then passed through 0.2 <mu> filter. In Step 4, the filtered media were added to the Schwann cell dishes prepared by Step 1. The effects were evaluated by follow-up count of Schwann cell numbers on photographs taken under phase contrast microscopy.
The results were that the most prominent Schwann cell proliferation was given by adding the media from 1 day culture of sciatic nerve with tellurium diet: Schwann cell increased to maximum value of +104% compared with just before adding. The media from other groups had lesser effect. These results suggested that the peripheral nerve in the process of Schwann cell proliferation as shown in tellurium neuropathy could contain or produce certain trophic factors for Schwann cells. Less
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Report
(1 results)
Research Products
(2 results)
