Cephalosporinase Gene Expression in E.coliK12
| Project/Area Number |
60570184
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
細菌学
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| Research Institution | Gunma University |
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Principal Investigator |
INOUE Matsuhisa Gunma University, Associate Professor, 医学部, 助教授 (10008336)
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| Project Period (FY) |
1985 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1986: ¥400,000 (Direct Cost: ¥400,000)
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| Keywords | cephalosporinase / inducible / constitutive / プラスミド / 宿主変異 |
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| Research Abstract |
The chromosomal cephalosporinase gene of gram-negative bacteria cloned on a small plasmid in Escherichia coli is expressed rather poorly.
Expression of this cephalosporinase gene on the recombinant plasmid was strikingly improved by mutation of a chromosomal gene of E.coli, designated,ded, which map on chromosomal gene of E.coli genetic map. The host mutation did not affect chromosomal beta-lactamase activity of E.coli, but it caused more than 50-fold stimulation of cephalosporinase production in the strains carrying cloned plasmid. A deletion derivative of cloned plasmid, which had lost a DNA sequence necessary for induction of the cephalosporinase, was unaffected on its expression by the deb mutation.
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Report
(1 results)
Research Products
(11 results)
