| Budget Amount *help |
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1986: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1985: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Research Abstract |
Hemolysin produced by Streptococcus faecalis has been shown to contribute to virulence in mice. Hemolysin production, clumping (pheromone) response, transferability of the hemolytic trait, structure of the hemolytic plasmid, and drug resistance were examined in 97 clinical isolates of S.faecalis in Japan.
The isolates were derived from various sources (i.e., urine, pus, vaginal, sputum, bile and blood), and approximately 60% were found to be hemolytic. About 85% of the hemolytic strains exhibited a clumping response compared to about 49% of the non-hemolytic strains. Over 50% of the hemolytic strains carried transferable hemolysin determinants, and in no case were drug-resistance genes linked. The hemolytic strains exhibited multiple drug-resistance more frequently than the non-hemolytic strains. In contrast to the high frequency of hemolysin producers among parenteral isolates, strains derived from fecal specimens of healthy individuals exhibited a low (17%) incidence of hemolysin production.
Hemolysin-bacteriocin plasmid pAD1(56.7kb), isolated in Ann Arbor, MI, is one of the well analyzed conjugative plasmids in S.faecalis. Eleven transferable cAD1-responding plasmids in the present study were found to structurally resemble (restriction fragments) pAD1.
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