| Project/Area Number |
60570211
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Virology
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| Research Institution | Sapporo Medical College |
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Principal Investigator |
URASAWA Shozo School of Medicine, Sapporo Medical College, 医学部, 教授 (00045379)
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| Co-Investigator(Kenkyū-buntansha) |
TANIGUCHI Koki School of Medicine, Sapporo Medical College, 医学部衛生学, 講師 (40094213)
URASAWA Tomoko School of Allied Health Professions, Sapporo Medical College, 衛生短期大学部看護学科, 教授 (90045378)
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| Project Period (FY) |
1985 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1986: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1985: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | Human rotavirus / Genetic reassortment / Serotype antigen / Subgroup antigen / Viral protein / ウィルスRNA |
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| Research Abstract |
Two reassortant viruses were selected from viral clones obtained by a mixed infection of MA104 cells with human rotavirus strains Wa (serotype 1-subgroup II) and HN126 (serotype 2-subgroup I),using either serotype 2 or serotype 1-specific antisera as selective pressure. Neutralization test with serotype-specific antisera and enzyme-linked immunosorbent assay with subgroup-specific monoclonal antibodies revealed that they were reassortant strains having unique antigenic compositions not yet encountered in clinical specimens, i.e. serotype 1-subgroup I (C116 strain) and serotype 2-subgroup II (C15 strain).
Analysis by polyacrylamide gel electrophoresis of viral RNA concluded that while RNA segment 6 of C116 coding for the major subgrouping protein VP6 was derived from HN126 parent, its segments 4 and 9 coding for the minor and major serotype-specific neutralizing proteins VP3 and VP7, respectively were derived from Wa parent. In contrast, the reassortant C15 was found to contain segment 6 (encoding subgrouping protein VP6) originated from Wa parent. Regarding RNAs encoding neutralizing proteins, C15, while receiving segment 4 coding for VP3 from Wa parent, contained the genome segment coding for VP7 (either segment 8 or 9) from HN126 parent. Although this serotypic mosaic strain in terms of its genomic constitution was shown to be a serotype 2 virus on the basis of its preferential reactivity in neutralization reaction with serotype 2 antiserum (possibly because of the major neutralizing protein VP7 of HN126 origin), surprisingly the antiserum against C15 equally neutralized both serotype 1 and serotype 2 strains. This result indicates the great utility of genetically reassorted strains as promissing candidates for rotavirus vaccine production.
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