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The Function of HLA Class II Gene Products Expressed by Gene Transfer

Research Project

Project/Area Number 60570217
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Immunology
Research InstitutionHamamatsu University School of Medicine

Principal Investigator

KOIDE YUKIO  Associate Prof. Hamamatsu University School of Medicine, Dept. of Microbiology/Immunology, 医学部, 助教授 (30126809)

Co-Investigator(Kenkyū-buntansha) HARAGUCHI Soichi  Assistant Prof. Same as above, 医学部, 助手 (00115502)
YOSHIDA Takato O  Prof. Same as above, 医学部, 教授 (90041847)
伊奈 康孝  名古屋市立大学, 医学部, 助手 (20168397)
Project Period (FY) 1985 – 1987
Project Status Completed (Fiscal Year 1987)
Budget Amount *help
¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1987: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1986: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1985: ¥1,000,000 (Direct Cost: ¥1,000,000)
KeywordsHLA class II antigens / Gene Transfer / MLR / 抗体産生 / HLA-DQgene
Research Abstract

HLA class II antigens play an essential role in the immune response by interacting with regulatory T cells. At least three distinct HLA class II antigens, designated HLA-DR, -DQ, and - DP,exist and are expressed simultaneously on the same cells. Therefore, it is extremely difficult to examine the role of each class II antigen in the immune response. We introduced alpha and beta chain genes of one of DR, DQ, and DP antigens into murine L cells and obtained transformants expressing DR, DQ, or DP antigens individually. Applying these transfectants to mixed lymphocyte reaction (MLR) and the antibody-production in MLR, we assessed the function of DR, DQ, and DP antigens individually. In MLR, the order of stimulating abilities appeared to be DR>DQ>DP antigen-expressing transformants. As the stimulating ability in MLR did not correlate with the antigen-dose, it seems likely that the different responsiveness in MLR did not reflect the difference of antigen-dose but reflected the size of T cell pool responding each of DR, DQ, and DP antigens.
MLR caused by these transformants were blocked by monoclonal antibodies against each class II antigens, indicating that these MLR were elicited by class II antigens expressed on transformants. Next, we assessed the role of class II antigens in the IgG and IgM-production in MLR. Similar to the results of MLR, the amount of antibody-production caused by the transformants was DR>DQ>DP-expressing transformants in both IgG and IgM. Mixing experiments of transformants showed that no suppressor T cells was induced by any transformants.
Taken together, these results indicate that the different responsiveness to transformants in both MLR and the antibody-production is attributable to the different sizes of T cell clone pools responding to each class II antigen.

Report

(2 results)
  • 1987 Final Research Report Summary
  • 1986 Annual Research Report

Research Products

(23 results)

All Other

All Publications (23 results)

  • [Publications] Nakatsuji,T.;Inoko,H.;Ando,A.;Sato,T.;Koide,Y.;Tadakuma,T.;Yoshida,TO,et: Immunogenetics. 25. 1-6 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Ina,Y.;Koide,Y.;Fujishima,Y.;Hori,T.;Inoko,H.;Ando,A,;Tsuji,K.Yoshida,To: HLA in Asia-Oceania 1986. 898-901 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Ueda,R.;Nishida,K.;Koide,Y.;Tsuge,I.;Seto,M.;Yoshida,M.;Miyoshi,I.;Ota,K.;Takahashi,T.: Cancer Res.45. 1314-1319 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Ina,Y.;Koide,Y.;Nezu,N.;Yoshida,TO.: The Journal of Immunology. 139. 1711-1717 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Koide,Y.;Ina,Y.;Nezu,N.;Yoshida,TO: Proc.Natl.Acad.Sci.USA.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Matsuo,T.;Ryu,K.;Koide,Y.;Yoshida,TO: Transplant.Proc.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Koide.Y.;Yoshida,T.O.: "Structural and functional aspects of HLA class II genes In "Comparative Aspects of Differentiation Antigens"" Marcel Dekker(N.Y.), 47-63 (1988)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Yoshida,To,Koide,Y.;Fujishima,Y.Hori,T.;Masamoto,T.;Wu,CL.;Haraguchi,S.;Nishimura,M.: "Gene modifications snd autoimmune mechanisms in NOD mouse strain as human IDDM model.In "New Horizons in Animal Models for Autoimmune Diseases"" Academic Press(N.Y.), 59-67 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Nakatsuji,T.,Inoko,H.,Ando,A.,Sato,T.,Koide,Y.,Tadakuma,T.,Yoshida,T.O.,Tsuji,K.: "The role of transfected HLA-DQ genes in the mixed lymphocyte-like condition" Immunogenetics. 25. 1-6 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Ueda, R.,Nishida,K.,Koide,Y.,Tsuge,I.,Seto,M.,Yoshida,M.,Miyoshi,I.,Ota,K., Takahashi,T.: "Two mouse monoclonal antibodies detecting different epitopes of an activate lymphocyte antigen on adult T cell leukemia cells" Cancer Res.45. 1314-1319 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Ina, Y., Koide,Y., Nezu,N., Yoshida,T.O.: "Regulation of HLA class II antigen expression: Intracellular signaling molecules responsible for the regulation by interferon- and crosslinking of Fc receptors" J. Immunol.139. 1711-1717 (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Koide, Y., Ina,Y., Nezu,N., Yoshida,T.O.: "Calcium influx and the consequent Ca^<2+>-calmodulin regulated process are involved in interferon- -induced expression of HLA class II molecules on HL-60" Proc.Natl.Acad.Sci.USA. in press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Matsuo, T., Ryu,K., Koide,Y., Yoshida, T.O.: "Establishment and characterization of U937-B cell line hybridoma expressing HLA class II molecules" Transplant. Proc.in press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Koide,Y.,Yoshida,T.O.: Structural and functional aspects of HLA class II genes. IN "Comparative Aspects of Differentiation Antigens". Marcel Dekker, 47-63 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1987 Final Research Report Summary
  • [Publications] Nakatsuji,Takako: Immunogenetics.

    • Related Report
      1986 Annual Research Report
  • [Publications] Iseki,Rieko: Microbiology and Immunology. 31. 59-74 (1987)

    • Related Report
      1986 Annual Research Report
  • [Publications] 原口惣一: 癌と化学療法. 13. 3236-3243 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] 伊奈康孝: 臨床免疫.

    • Related Report
      1986 Annual Research Report
  • [Publications] 伊奈康孝: 臨床免疫.

    • Related Report
      1986 Annual Research Report
  • [Publications] Nakatsuji,Takako: "Expression of transfected HLA-DQ gene and its functional ability in MLR.In"HLA IN ASIA-OCEANIA"" M.Aizawa(Hokkaido University Press), 1140 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] Koide,Yukio: "Structural and functional aspects of HLA class 【II】 genes In"Comparative aspects of differentiation antigens of hemopoietic cells"" M.Miyasaka and Z.Trnka(Marcel Dekker),

    • Related Report
      1986 Annual Research Report
  • [Publications] Ina,Yasutaka: "Suppression of interferon(IFN)-γ-induced HLA class 【II】 antigens by crosslinking of surface Fc receptors.In"HLA IN OCEANIA 1986"" M.Aizawa(Hokkaido University Press), 1140 (1986)

    • Related Report
      1986 Annual Research Report
  • [Publications] 小出幸夫: "HLA遺伝子と抗原発現"HLAハンドブック"" 辻公美(サイエンス・フォーラム),

    • Related Report
      1986 Annual Research Report

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Published: 1987-03-30   Modified: 2016-04-21  

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