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Production of vascular permeability factors in minimal change nephrotic syndrome by human T cell hybridoma.

Research Project

Project/Area Number 60570284
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 内科学一般
Research InstitutionINSTITUTE OF CLINICAL MEDICINE, UNIVERSITY OF TSUKUBA

Principal Investigator

TOJO Shizuo  Professor, Institute of Clinical Medicine, University of Tsukuba, 臨床医学系, 教授 (20009103)

Co-Investigator(Kenkyū-buntansha) KOYAMA Akio  Associate professor, Institute of Clinical Medicine University of Tsukuba, 臨床医学系, 助教授 (80111384)
NARITA Mitsuharu  Professor Institute of Clinical Medicine, University of Tsukuba, 臨床医学系, 教授 (80009472)
Project Period (FY) 1985 – 1986
Project Status Completed (Fiscal Year 1986)
Budget Amount *help
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1986: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1985: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsMinimal Change Nephrotic Syndrome / Vascular permeability factor / T cell Hybrydoma / Lynphokine
Research Abstract

The pathogenesis of minimal change nephrotic syndrome(MCNS) is still unclear. Recently there were several reports that T cell dysfunction was observed, associated with the onset of MCNS and a kind of "lymphokine" might cause abnormal increase in the glomerular vascular permeability.
In this study, we investigated the characteristics of the factors which cause the glomerular vascular permeability by the production of lymphokines using human T cell hybridoma.
The lymphocytes from patients with MCNS were hybridized with CCRF-HSB2 cells, by polyethyleneglycole method. The factors which produce urinary protein was assayed by the iv injection of the culture supernatants into rats and examined the effects of lymphokines on IL-II dependent MTH mouse cell line, ConA-induced lymphocyte blast formation, grouth of cell lines from hematogenous organ and carcinoma.
We observed factor(s) which caused significant urinary proteins and also enhanced normal lymphocye grouth (more than 10 times) in ConA-induced lymphocte blast formation. The supernatants also showed cytotoxic effect on carcinoma cell lines. However, during cloning, the activities of the factor decreased.
We supeculated that thevvascular permeability factor and T cell activating factor might be a lymphokine which T cell produced in patients with MCNS, and play an important role in pathogenesis of MCNS.

Report

(1 results)
  • 1986 Final Research Report Summary

Research Products

(5 results)

All Other

All Publications (5 results)

  • [Publications] Koyama,A;Inage,H.;Narita,M.;Tojo,S.: Immunolgy. 58. 533-540 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Koya Narita,M;Tojo,S.: Immunology. 58. 529-534 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Akio KOyama, Mitsuharu Narita, Shizuo Tojo;: "Vascular permeability factor in patients with minimal change nephrotic syndrome produced by T cell hybrydoma." Immunology. 58. 533-540 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] AKIO KOYAMA, INAGE,H., KOBAYASHI,M., NARITA,M. TOJO,S.: "EFFECT OF CHEMICAL MODIFICATION OF ANTIGEN ON THE CHARACTERISTICS OF IMMUNE COMPLEXES AND THEIR GLOMERULAR LOCALIZATION IN THE MURINE RENAL TISSUES." IMMUNOLOGY. 58. 529-534 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] KOYAMA,A., INAGE,H., KOBAYASHI,M., NAKAMURA, H., NARITA,M., TOJO,S.: "EFFECT OF CHEMICAL CATIONIZATION OF ANTIGEN ON GLOMERULAR LOCALIZATION OF IMMUNE COMPLEXES IN ACTIVE MODELS OF SERUM SICKNESS IN RABBITS."

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary

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Published: 1987-03-31   Modified: 2016-04-21  

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