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Evidence for detection of antibody directed against collagen-like region of Clq in sera from patients with systemic lupus erythematosus and its pathogenic significance in lupus nephritis

Research Project

Project/Area Number 60570288
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field 内科学一般
Research InstitutionUniversity of Tokyo

Principal Investigator

AIZAWA Chikara (1986)  First Department of Intern. Med., Facuty of Med.,Univ. of Tokyo, 医学部, 助手 (70111490)

上床 周 (1985)  東京大学, 助手

Co-Investigator(Kenkyū-buntansha) AOTSUKA Shinichi  Division of Immunology, Clinical Research Institute, National Medical Center, 臨床研究部, 室長 (80158716)
Project Period (FY) 1985 – 1986
Project Status Completed (Fiscal Year 1986)
Budget Amount *help
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1986: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1985: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsClq / Clq collagen-like region / Clq globular portion / Clq solid-phase radioimmunoassay / Systemic lupus erythematosus (SLE) / Pepsin digestion / Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) / ウェスタンブロッティング法
Research Abstract

In earlier studies we showed that the Clq-binding IgG in the sera from patients with systemic lupus erythematosus (SLE) tested by Clq solid-phase radioimmunoassay is cofractionated with monomeric IgG on gel filtration and mostly binds to Clq via the F(ab')2 region. In this study, we found that Clq, even when stripped of its immune complex-binding globular regions by pepsin digestion, retained a substantial part of its ability to bind IgG from SLE sera, suggesting that the collagen-like region of Clq is involved in binding to the SLE IgG. Heat-inactivation of Clq also failed to abolish its ability to bind IgG from SLE sera. In contrast, the binding of Clq to heat-aggregated IgG was completely abrogated by these treatments. In addition, the reaction of heat aggregated IgG with the solid-phase Clq was markedly dependent on ionic strength whereas the binding of IgG from SLE sera with the solid-phase Clq persisted by high concentrations of salt. These findings suggest that the Clq-binding IgG in SLE sera is, at least in part, antibody directed against the collagen-like region of Clq.
SDS-PAGE and Western blotting analysis could revealed the binding of SLE IgG neither to the collagen-like region nor the globular portion of Clq, suggesting that the tertially structural conformation may be essential to express its antigenicity.
Cross-reactions of anti-Clq antibodies to various types of collagen or glomerular basement membrane (GBM) are now under investigation.
lupus nephritis

Report

(1 results)
  • 1986 Final Research Report Summary
  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] 上床周,青塚新一,大川雅子,江草康夫,横張龍一,相澤力,鈴木慶治: Clin.Exp.lmmunol.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 青塚新一: リウマチ.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Uwatoko S, Aotsuka S, Okawa M, Egusa Y, Yokohari R, Aizawa C, and Suzuki K: "Clq solid-phase radioimmunoassay: Evidence for detection of antibody directed against collagen-like region of Clq in sera from patients with systemic lupus erythematosus and its pathogenic significance in" Clin. Exp. Immunol.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Aotsuka S.: "Cross-reactivity of anti-DNA antibodies." The Ryumachi.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary

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Published: 1987-03-31   Modified: 2016-04-21  

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