| Project/Area Number |
60570301
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
内科学一般
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| Research Institution | KEIO UNIVERSITY SCHOOL OF MEDICINE |
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Principal Investigator |
TOJO TAKESHI KEIO UNIVERSITY SCHOOL OF MEDICINE Assistant Professor, 医学部, 講師 (40051362)
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| Co-Investigator(Kenkyū-buntansha) |
KABURAKI JUNICHI KEIO UNIVERSITY SCHOOL OF MEDICINE, 医学部, 助手 (40175287)
OKANO YUTAKA KEIO UNIVERSITY SCHOOL OF MEDICINE, 医学部, 助手 (80160696)
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| Project Period (FY) |
1985 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1986: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1985: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | ANTINUCLEAR / ANTIBODY / AUTOANTIBODY / NONHISTONE / NUCLEOPROTEIN |
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| Research Abstract |
Recent studies have shown that antinuclear antibodies (ANAs) in sera of patients with various connective tissue diseases are directed against variety of non-histone nucleoproteins.
In double immunodiffusion tests using rabbit thymus extract as the antigen, 472 sera from patients with various collagen diseases were screened for precipitating antibodies to non-histone nucleoproteins. Twenty-three sera(5%) showed undifferentiable precipitine lines. From these precipitating antibodies, a new antigen-antibody system named Fu system was described. The Fu antigen was a non-histone nucleoprotein not containing RNA or DNA. All three patients with anti-Fu antibody were diagnosed as SLE.
In immunoprecipitation assay using <^(32)P> -labelled nucleoproteins 341 sera from patients with collagen diseases were screened for antinuclear antibodies against small molecular RNA containing nucleoproteins(snRNPs). The antibodies were detected in 159/341 sera tested. These antibodies could be classified into three types by combination of snRNPs precipitated : anti-U1RNP antibody only, anti-(U1,2,4-6) RNP(Sm) antibody and anti-(U1,U2)RNP antibody. There were 13 sera containing anti-(U,U2)RNP antibody. Patients with the antibody were diagnosed as overlap syndrome in 7, PSS in 5, PM/DM in 1.
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