| Project/Area Number |
60570317
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Gastroenterology
|
|---|
| Research Institution | Nagoya University |
|---|
Principal Investigator |
KAKUMU Shinichi Nagoya University School of Medicine, 医学部, 助手 (10115545)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
田原 裕文 名古屋大学, 医学部, 医員
藤 明彦 名古屋大学, 医学部, 医員
広藤 秀雄 名古屋大学, 医学部, 医員
大谷 由幸 名古屋大学, 医学部, 医員
FUJI Akihiko Nagoya University School of Medicine
TAHARA Hirofumi Nagoya University School of Medicine
OHTANI Yoshiyuki Nagoya University School of Medicine
HIROFUJI Hideo Nagoya University School of Medicine
|
|---|
| Project Period (FY) |
1985 – 1986
|
| Project Status |
Completed (Fiscal Year 1986)
|
| Budget Amount *help |
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1986: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1985: ¥1,500,000 (Direct Cost: ¥1,500,000)
|
|---|
| Keywords | anti-HBs production / human peripheral blood mononuclear cells / purified HBsAg / anti-idiotypic antibody / interleukin 2 / pokeweed mitogen / ポークウィードマイトロジェン |
|---|
| Research Abstract |
1. Enhancement of antibody production to hepatitis B surface antigen by anti-idiotypic antibody. Studies were undertaken to determine whether anti-idiotypic antibody against antibody to hepatitis B surface antigen (anti-HBS) could modulate in vitro anti-HBS production by human peripheral blood mononuclear cells stimulated with pokeweed mitogen. Peripheral blood mononuclear cells from patients positive for serum anti-HBs produced significantly increased amounts of anti-HBs by the addition of F <(ab')_2> fragments of anti-anti-HBs as well as purified HBsAg when compared to those in cultures with pokeweed mitogen alone, indicating that anti-idiotypic antibody modulates the immune response to HBsAg.
2. In vitro synthesis of antibody to hepatitis B virus antigen by circulating lymphocytes from chronic HBsAg carriers and patients with acute hepatitis B. The synthesis of IgG, anti-HBs and anti-HBc was measured in pokeweed mitogen-stimulated cultures of peripheral blood mononuclear cells from chronic HBsAg carriers, patients with acute hepatitis B (HAB) and subjects with anti-HBs in serum (controls). Coculture experiments indicated that decreased B cell function and increased suppressor T cell function specific for HBsAg contributed to the lack of anti-HBs in serum of chronic HBsAg carriers and AHB patients during the recovery phase.
3. Enhancement of antibody production to hepatitis B surface antigen by interleukin 2. We studied to assess whether induction of anti-HBs production by cultured lymphocytes which had been conducted with pokeweed mitogen could be replaced by interleukin 2 (IL2). In cultures stimulated with purified HBsAg and IL2, the comparable levels of anti-HBs production to those obtained in cultures stimulated with pokeweed mitogen alone occured when peripheral blood mononuclear cells from patients positive for serum anti-HBs and recipients of HBsAg vaccine were used as effector cells. However, IL2 alone did not induce anti-HBs production.
|
