| Project/Area Number |
60570324
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Gastroenterology
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| Research Institution | Shimane Medical University |
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Principal Investigator |
SHIMADA Yoshihiro Shimane Medical University School of Medicine, 医学部, 教授 (50032868)
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| Co-Investigator(Kenkyū-buntansha) |
IKEDA Satoru Shimane Medical University School of Medicine, 医学部, 助手 (60151289)
NISHIMURA Koichi Shimane Medical University School of Medicine, 医学部, 助手 (30144702)
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| Project Period (FY) |
1985 – 1987
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| Project Status |
Completed (Fiscal Year 1987)
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| Budget Amount *help |
¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1987: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1986: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1985: ¥800,000 (Direct Cost: ¥800,000)
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| Keywords | DHBV / Immuno regulatory drugs / Carrier / Steroid / OK-432 / HBキャリアー / ステロイドホルモン |
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| Research Abstract |
To investigate the relationship among the time of viral infection, infection pattern and lever diseases experimental transmission of Duck hepatitis B virus (DHBV) was performed. Inoculation of DHBV into ducks on 1 day and 3 day posthatch revealed persistent infection, whereras inoculation into those on and after 5 day posthatch showed transient viremia. Hepatitis activity was seen in ducks inoculated with DHBV on and after 3 day posthatch and was very weak compared with HBV associated liver diseases. Second, alteration of infection pattern of DHBV by immuno regulatory drugs was investigated. Immunosuprresive treatment with cyclophosphamide extended viremic period and showed almost no hepatic inflammation. Treatment of steroid did not suppress the hepatitis and moreover increased viral replication and extended viremic period. Immuno stimmulation treatment with OK-432 showed increase in hepatic inflammation but did not increase viral replication. Alterations of infection pattern of DHBV with these drugs were quite similar to that of HBV. Host immune response seems to have a considerable role on determination of infection pattern and activity of hepatic inflammation. With these results DHBV can be a helpful model of HBV for studying host-viral interaction and immunological mechanism of viral hepatitis.
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