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A research on shared antigen between myelin-associated glycoprotein and lymphocytes

Research Project

Project/Area Number 60570364
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Neurology
Research InstitutionNiigata University

Principal Investigator

SATO Shuzo  Hospital of Medical School, Niigata University, 医学部, 助手 (30115034)

Co-Investigator(Kenkyū-buntansha) MIYATAKE Tadashi  Professor, Niigata University, 脳研究所, 教授 (50048998)
Project Period (FY) 1985 – 1986
Project Status Completed (Fiscal Year 1986)
Budget Amount *help
¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1986: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1985: ¥900,000 (Direct Cost: ¥900,000)
Keywordsmyelin / glycoprotein / myelin-associated glycoprotein (MAG) / monoclonal antibody / natural killer cell / multiple sclerosis / Guillain-Barre syndrome / 癌
Research Abstract

Myelin-associated glycoprotein is a minor constituent of myelin and has a shared antigen with human lymphocytes especially with natural killer (NK) cells. We have completed followings on this research field.
1. We have generated 19 kinds of anti-MAG monoclonal antibodies and examined those characters. There groups of antibodies were indentified: IgG antibodies recognizing the polypeptide moiety and IgG and IgM antibodies recognizing the carbohydrate moiety of MAG molecule. Relating with anti-Leu-7 2 groups were identified, one has similar and the other has slightly defferent antigenic specificity with anti-Leu-7.
2. Antigenic protein molecules in HSB-2 cells (immunogen of anti-Leu-7) and lymphocytes in peripheral blood were examined using monoclonal anti-MAG antibodies. Several protein molecules were recognized on immunoblots.
3. An anti-MAG monoclonal antibody which recognized carbohydrate part of MAG inhibited NK activity in vitro.
4. We developed ELISA system for detection of anti-MAG antibody and found some patients with multiple sclerosis and Guillain-Barre syndrome have elevated anti-MAG titer.
5. We also developed ELISA system for quantitation of MAG using 2 kinds of monoclonal antibodies.
6. We showed a cross antigenicity between MAG and lung cancer using a monoclonal anti-MAG antibody immunohistochemically.

Report

(1 results)
  • 1986 Final Research Report Summary

Research Products

(13 results)

All Other

All Publications

  • [Publications] Shuzo Sato et al.: "Shared antigen between the myelin-associated glycoprotein (MAG) and a cell line from human T cell leukemia (HSB-2)." Journal of Neuroimmunology. 7. 287-298 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Masami Tanaka et al.: "Human natural killer cell activity is reduced by treatment of anti-myelin-associated glycoprotein (MAG) monoclonal mouse IgM antibody and complement." Journal of Neuroimmunology. 10. 115-127 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Masami Tanaka et al.: "Human peripheral lymphocytes defined by anti-myelin-associated glycoprotein antiserum in healthy individuals and in patients with multiple sclerosis." Acta Neurologica Scandinavica. 71. 278-283 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Masami Tanaka et al.: "Subsets of human natural killer cells in neurological disorders." Clinical Neurology. 25. 314-319 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Shuzo Sato et al.: "Anti-myelin-associated glycoprotein antibody in sera from patients with demyelinating disease." Acta Neurologica Scandinavica. 74. 115-120 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Hiroko Baba et al.: "Characterization of the antigenic determinant on HSB-2 cells shared with myelin-associated glycoprotein (MAG) using monoclonal antibodies." Journal of Neuroimmunology. 13. 89-97 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Masatoyo Nishizawa et al.: "Production and characterization of monoclonal antibodies against myelin-associated glycoprotein." Journal of Neurochemistry. 47. 1893-1900 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Masami Tanaka et al.: "Chronic T cell leukemia with a NK phenotype reacting with anti-myelin-associated glycoprotein (MAG) mouse monoclonal antibody." Tohoku Journal of experimental Medicine. 150. 225-226 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Masami Tanaka et al.: "Human natural killer cells stained by anti-myelin-associated glycoprotein (MAG) antiserum and circulating anti-MAG IgM antibody in patients with neurological disorders." Neurological Medicine. 25. 601-603 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Motomi Arai et al.: "Murine monoclonal antibodies to the myelin-associated glycoprotein (MAG) recognize Leu 7 reactive molecules on human mononuclear cells." Journal of Immunology.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Isao Hozumi et al.: "Shared carbohydrate antigenic determinant between the myelin-associated glycoprotein (MAG) and lung cancer. An immunohistochemical study by an anti-MAG IgM monoclonal antibody." Journal of Neuroimmunology. in press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Hiroko Baba et al.: "Developmental changes of myelin-associated glycoprotein in rat brain: Study on experimental hyperphenylalanimeia." Neurochemical Research. in press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Nobuyuki Miyatani et al.: "Antibody to sialosyllactosaminylparagloboside in a patient with IgM paraproteinemia and polyradiculoneuropathy." Journal of Neuroimmunology. in press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary

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Published: 1987-03-30   Modified: 2016-04-21  

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