Ultrastructural Localization of Specific Proteins in Nerve and Muscle Cells from Patients with Neuromuscular Diseases with Colloidal Gold Method
| Project/Area Number |
60570373
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Neurology
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| Research Institution | Juntendo University |
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Principal Investigator |
ANNO Midori Juntendo University School of Medicine, Associate, 医学部, 助手 (90138256)
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| Co-Investigator(Kenkyū-buntansha) |
KOMIYA Tadatoshi Juntendo University School of Medicine, Associate, 医学部, 助手 (90170381)
SATO Takeshi Juntendo University School of Medicine, Associate Professor, 医学部, 助教授 (40018594)
YAMAMOTO Takeshi Juntendo University School of Medicine, Associate
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| Project Period (FY) |
1985 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1986: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1985: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | Immuno-electron microscopy / mitochondria / myopathy / cytochrome c oxidase / mitochondrial encephalomyopathy / measles virus |
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| Research Abstract |
1. Colloid-gold labelling of mitochondrial enzymes for immuno-electron microscopy:
Ultrastructural localization of mitochondrial electron-transport enzymes, complex <I> , <III> , and <IV> was accomplished by immunocytochemistry on methacrylate embedded muscle sections. Results showed a very distinct and dense labelling of complex <I> , <III> , and <IV> , respectively in the inner membrane of mitochondria. Whereas a few gold particles were present in matrix. Labelling on the paracrystalline inclusions was substantially weaker in abnormal giant mitochondria, but gold particles were extensively confined on the membrane of cristae surrounding the inclusions.
2. Immunocytochemical demonstration of complex <III> and <IV> in tissues from patients with mitochondrial encephalomyopathy:
Mitochondria were specifically stained in sections of formalin-fixed paraffin-embedded human tissues by ABC method. A autopsy case with mitochondrial encephalomyopathy showed a deficiency of complex <IV> (cytochrome c oxidase) in skeletal muscle only.
3. Immunocytochemical studies on SSPE virus:
Immuno-electron microscopic studies on SSPE virus infected cells and brain tissues were carried out using monoclonal antibodies against measles virus polypeptides. The nucleocapsids in the dendrites of hamster infected with measles virus were stained with monoclonal antibody against NP.
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Report
(1 results)
Research Products
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