| Project/Area Number |
60570380
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | TOHOKU UNIVERSITY |
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Principal Investigator |
KIMURA Tokihisa The Second Department of Internal Medicine, Tohoku University School of Medicine, 医学部, 助手 (00004945)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUI Kuniaki The Second Department of Internal Medicine, Tohoku University School of Medicine, 医学部附属病院, 助手 (40125654)
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| Project Period (FY) |
1985 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1986: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1985: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | atrial natriuretic peptide / renin / vasopressin / natriuresis / diuresis / レニン-アンギオテンシン / アルドステロン |
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| Research Abstract |
In order to investigate effects of centrally or peripherally administered atrial natriuretic peptide (ANP) on the renin-angiotensin-aldosterone system, vasopressin (AVP) release and renal function, experiments were carried out in dogs with anesthetized with pentobarbital. <alpha> -hANP (human ANP) was administered intracerebroventricularly (icv) or systematically. Moreover, to assess the mutual relationship between ANP and AVP in man, the sensitive radioimmunoassay for human ANP was developed and plasma AVP and ANP levels were simultaneously determined under various hydrational states.
1) Effects of human atrial natriuretic peptide on renal function and vasopressin release: Systemic infusion of <alpha> -hANP (0.08 <micro> g・ <Kg^(-1)> ・ <min^(-1)> ) for 40 min caused a natriuresis and kaliuresis with a significant increase in renal blood flow (RBF) without changes in glomerular filtration rate (GFR) despite a decrease in mean arterial blood pressure (MABP). Plasma renin activity (PRA),
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aldosterone concentration (PAC) and AVP concentration were not changed significantly throughout the study. Therefore, it is concluded that ANP may increase RBF or affect directly the renal tubules, leading to a natriuresis.
2) Effects of centrally administered atrial natriuretic peptide on renal function: <alpha> -hANP given icv (8 ng・ <Kg^(-1)> ・ <min^(-1)> ) for 30 min caused a natriuresis and kaliuresis associated with a rise in RBF and a fall in plasma AVP, but plasma <alpha> -hANP, PRA and PAC and MABP were not change significantly. These results suggest that ANP in the brain may participate in the regulation of renal water and electrolyte handling via the unknown mechanisms.
3) Effects of acute water load, hypertonic saline infusion, and furosemide administration on atrial natriuretic peptide and vasopressin releases in humans: Plasma <alpha> -hANP rose in response to the expansion of plasma volume due to acute water load or hypertonic saline infusion and fell due to the contraction of plasma volume. AVP was changed mainly in response to changes in plasma osmolality. Less
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