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Serum Lipoproteins in Normal Arterial Tissues in Man

Research Project

Project/Area Number 60570407
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Circulatory organs internal medicine
Research InstitutionDepartment of Medicne, Keio University School of Medicine

Principal Investigator

HATA Yoshiya  Department of Medicine, Keio University School of Medicne, 医学部, 講師 (70051258)

Co-Investigator(Kenkyū-buntansha) ISHII Toshiharu  Department of Pathology, Keio University School of Medicine, 医学部病理, 講師 (30101893)
FUKUZAWA Tsunetoshi  Department of Medicine, Keio University School of Medicine, 医学部内科, 助手 (40146593)
OIKAWA Takamitsu  Department of Medicine, Keio University School of Medicine, 医学部内科, 助手 (20129357)
YAMAUCHI Yoshio  Department of Medicine, Keio University School of Medicine, 医学部内科, 助手 (20129711)
YAMAMOTO Minoru  Department of Medicine, Keio University School of Medicine, 医学部内科, 助手 (30129720)
Project Period (FY) 1986
Project Status Completed (Fiscal Year 1986)
Budget Amount *help
¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1986: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1985: ¥1,200,000 (Direct Cost: ¥1,200,000)
KeywordsApolipoproteins / Serum lipoproteins / Tissue fluid lipoproteins / Normal intima / Foam cell / 泡沫細胞 / 動脈硬化
Research Abstract

In studying the relation between serum lipoporteins and intimal cells of normal arterial tissues in man, we investigated lipoproteins recovered from interstitial fluid of the tissue. We examined 26 thoracic aortas from autopsied subjects. Grossly normal intima was viewed by immunofluorescence and specific florescence to apolipoprotein A- <I> ,A- <II> , B, C- <II> , C- <III> , and E was observed. The remaining tissues were homogenized in Tris-HCL buffer at 4゜C for 3 minutes. The supernatants were applied to SDS gradient gel and PAG disc electrophoresis. They revealed apoprotein spots and lipoproetin bands corresponding to serum counterparts.The background density of the supernatents were adjusted to 1.006 and 1.063 to ultracentrifugically isolate VLDL and LDL. They were negatively stained with 2& glutaraldehyde, viewed under TEM. There were spheres equivalent to serum lipoproteins of VLDL and LDL in size and shape. The lipoproein fractions separated from aortic tissue fluids were furthe … More r fixed with 1% Os <O_4> ,overnight, then examined under SEM. It also showed small particles similar to serum counterparts. The remaining supernatants were further used for quntitative measurements of apolipoproteins. They were dialysed and condensed under vacum to apply for SRID of apoproteins. Serum total cholesterol measured just before death was correlated to tissue apoproteins. Apo B concentrations in normal intima were 1/7 to 1/10 of serum apo B, tissue apoA- <I> and A- <II> were about 1/50, tissue apoE 1/3 of serum counterparts, respectively.
These indicated that there occur serum lipoproteirs of VLDL and LDL in normal intima of aorta, probably solubilized in interstitial fluid of intimal tissue. The lipoprotein concentrations were not equal to serum, but had a gradient between tissue and serum, about 1/10 for LDL and 1/50 for HDL. These difference in lipoprotein concentrations may constitute an internal environment for intimal cells, and their change may be causal for the formation of foam cells in the intimal tissues. We plan to analyse the change in lipoprotein concentrations in atherosclerotic lesions. Less

Report

(1 results)
  • 1986 Final Research Report Summary

Research Products

(15 results)

All Other

All Publications (15 results)

  • [Publications] 秦葭哉: 脈管学. 23. 563 (1983)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 秦葭哉: 動脈硬化. 16(抄). 18 (1984)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Hata,Y.& Nakajima,K.: Proceedings of 7th International Symposium on Atherosclerosis. 7. 104-105 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 秦葭哉,中島久実子: 最新医学. 39. 1839-1845 (1984)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 秦葭哉: Clinician. 81. 487-492 (1984)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 秦葭哉,中島久実子: Therapeutic Research. 3. 101-109 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 秦葭哉,中島久実子: 脈管学. 25. 247-254 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 秦葭哉,中島久実子: 61年度「血管代謝」研究報告集. 1-4 (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 秦葭哉,中島久実子,山本実,山内喜夫,石井寿晴: 動脈硬化.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Y.Hata;K.Nakajima: J.Clin Biochem Nutr.2.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Hata,Y, & Nakajima,K.: "Serum Lipoproteins and Vascular Tissues" Proc. 7th Int,Symp.Atheroscler.7. 104-105 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Hata,Y. & Nakajima, K.: "Lipoprotein Size and Atherosclerosis" Therap. Res.3. 443-451 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Hata,Y. & Nakajima, K.: "Serum Lipoproteins in the Normal Arterial Tissues" J. Jpn Coll. Angiology. 25. 247-254 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Hata,Y & Nakajima,K.: "Serum and Arterial Tissue Lipoproteins" Saishin Igaku. 39. 1839-1845 (1984)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Hata, Y. & Nakajima.K.: "Serum Lipoprotein Concentrations in Normal Intima of Human Aorta" J. Clin. Biochem. Nutr.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary

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Published: 1987-03-30   Modified: 2016-04-21  

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