Study of thyroid hormone effects on developing brain and skeletal muscle using neonatal hypothyroid rat
| Project/Area Number |
60570434
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Pediatrics
|
|---|
| Research Institution | Department of Pediatrics, Hamamatsu University School of Medicine |
|---|
Principal Investigator |
IGARASHI Yoshio Professor, Department of Pediatrics, Hamamatsu University School of Medicine,, 医学部, 教授 (90010181)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
鶴井 聡 浜松医科大学, 医学部, 医員
杉江 陽子 浜松医科大学, 医学部, 医員
NISHIDA Mitsuhiro Assistant, Department of Pediatrics, Hamamatsu University School of Medicine,, 医学部, 助手 (70164564)
SUGIE Hideo (SUGIE YOKO) Assistant Professor, Department of Pediatrics, Hamamatsu University School of Me, 医学部, 講師 (60119980)
TSURUI Satoshi Clinical fellow, Department of Pediatrics, Hamamatsu University School of Medici
|
|---|
| Project Period (FY) |
1985 – 1987
|
| Project Status |
Completed (Fiscal Year 1987)
|
| Budget Amount *help |
¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1987: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1986: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1985: ¥600,000 (Direct Cost: ¥600,000)
|
|---|
| Keywords | thyroid hormone / cretinism / muscle histochemistry / brain development / protein synthesis / ミエリネーション / 甲状腺機能低下症 / 動物モデル / カルシウムポンプ / 筋小胞体 |
|---|
| Research Abstract |
Thyroid hormonal effects on developing rat brain and skeletal muscle were investigated using PTU induced neonatal hypothyroid rats. Morphological analysis using rat soleus muscle revealed disrupted histochemical maturation including the delayed maturation of type 1/type 2 fibermosaicism, the prolonged postnatal existence of myotubes, maldevelopment of fiber size and decreased number of total muscle cells. Schiatic nerve also showed the maturational delay, in which the disturbance of elongation of internodal length during development was evident, although the diameter of myelinated and unmyelinated nerve fibers were not significantly reduced. Brain development apparently affected by a lack of thyroid hormone, regarding the brain weight, DNA/RNA contents, protein contents and protein synthesis. These data suggest that the hypothyroid state on developing rat brain produces a disturbed cell multiplication and delayed growth of cell sizes which would be attributable to the decreased rate of protein synthesis. A locus for explaining this decreased rate of protein synthesis would be in tranalational process, especially in "ELONGATION" step in protein. Future study as to other hormonal effects, which is influenced by the thyroid hormone depletion, to the brain development, would be of interest.
|
Report
(2 results)
Research Products
(12 results)
