| Project/Area Number |
60570470
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Dermatology
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| Research Institution | Teikyo University |
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Principal Investigator |
MIZOGUCHI Masako Department of Dermatology, Teikyo University School of Medicine, 医学部, 教授 (30010250)
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| Co-Investigator(Kenkyū-buntansha) |
CHIKAKANE Kenichiro Department of Dermatology, Teikyo University School of Medicine, 医学部皮膚科, 助手 (30147079)
FURUSAWA Shuichi Department of Dermatology, Teikyo University School of Medicine, 医学部皮膚科, 助手 (80130037)
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| Project Period (FY) |
1985 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1986: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1985: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | atopic dermatitis / interleukin-1 / マクロファージ |
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| Research Abstract |
Patients with atopic dermatitis (AD) exhibit skin infection susceptibility and defective delayed type hypersensitivity. In order to study the relationship between these immune abnormalities and macrophage functions, we studied interleukin 1 (IL-1) which is a macrophage-derived protein and modulates many immune responses, such as host defense against infection or T cell proliferation through the induction of interleukin 2. Culture supernatants of lipopolysaccharide-stimulated peripheral blood monocytes (macrophages) from 12 AD patients and 10 age matched normal controls were used as IL-1 sources. High pressure liquid chromotography-purified IL-1 (culture supernatant) was also used. IL-1 activity was assayed by adding the supernatants to cultures of C3H/He.J mouse thymocytes (1.5 x <10^6> cells/well) with phytohemagglutinin (PHA) for 72 hrs. After a 6- hr pulse with ( <^3H> ) thymidine, cells were harvested and counted for incorporation of radioactivity. The mean value of IL-1 activity (stimulation index (SI)7.97 <-!+> 3,48 ) from macrophages of AD patients was significantly lower than that (SI = 11.68 <-!+> 2.80) of normal controls. Moreover, IL-1 activity correlated well with the severity of AD. Lymphocyte proliferation assays in vitro in the presence of PHA, Con A or SAC were performed, and the results were compared to IL-1 activity. IL-1 activity correlated with lymphocyte proliferation stimulated with PHA or Con A (T-cell stimulator), but not SAC (B cell stimulator). These findings suggest that macrophage dysfunction cause low IL-1 activity which may account for defective T-cell function in atopic dermatitis.
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