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Studies of porphyrin metabolic disorder and early diagnosis in porphyria with photodermatological manifestation.

Research Project

Project/Area Number 60570473
Research Category

Grant-in-Aid for General Scientific Research (C)

Allocation TypeSingle-year Grants
Research Field Dermatology
Research InstitutionThe Institute of Public Health

Principal Investigator

KONDO Masao  The Institute of Public Health, その他, その他 (80111110)

Project Period (FY) 1985 – 1986
Project Status Completed (Fiscal Year 1986)
Budget Amount *help
¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1986: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1985: ¥800,000 (Direct Cost: ¥800,000)
KeywordsPorphyria / Diagnosis / High-Performance Liquid Chromatography / Porphyrin metabolism / Porphyria cutanea tarda / Erythropoietic protoporphyria / Variegate porphyria / 先天性ポルフィリン症
Research Abstract

1. The development of method in early stage of porphyria. I established a method of simultaneaus measurement of urinary, pheripheral blood, and fecal porphyrins by high-performance liquid chromatography. This method can be used as a routine method for mass screening of diagnosis in patients with porphyria.
2. The mechanism of abnormalities of porphyrin metabolism in four types of porphyria with photodermatological manifestation.
Approximately 60 cases in porphyrias obtained were subjected to the clinical and biological investigations, in which the activities of five enzymes out from the eight heme biosynthetic enzymes and also the content of porphyrins and two precursors, ALA and PBG in the bioptic materials from liver, bone marrow and peripheral blood were determined. Several methods and experimental techniques were newly devised or developed during this study.
Hepatic ALA dehydratase activity in PCT patients were markedly decreased. Summarizing the present experiments on ALA dehydratase enzyme, the decrease activity to be caused by a decreased in the enzyme protein. From the present rat experiments, it was found that alcohol, estrogen and hexachlorobenzene treated rats showed no change in hepatic ALA dehydratase activity compared to controls, postulating an enzymatic discrepancy between PCT patients and experimental PCT.
Two cases that markedly decreased of erythrocyte URO'gen decarboxylase activity was found in PCT patients.
The enzymes activities and porphyrin metabolites of heme biosynthesis were determined in 15 patients in EPP, 6 cases of VP and 7 cases of CP. As the results, ALA synthase activity was found to be markedly increased in VP. Furthermore, author made a basic data for diagnosis of porphyrias.
3. The clinical statistics in porphyrias. All the cases of porphyria ever reported in Japan were collected and subjected to clinical statistics and epidemiological observation in order to summarize the current informations about porphyrias in Japan.

Report

(1 results)
  • 1986 Final Research Report Summary
  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] 近藤雅雄: 日本皮膚科学会雑誌. 95. 995-996 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Masao KONDO;Gumpei URATA: Clinical Chemistry. 31. 427-429 (1985)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 近藤雅雄,鈴木妙子: 公衆衛生院研究報告. 35. (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] 近藤雅雄: LC family. 22. (1987)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Masao KONDO;Yoshitaka SHIMIZU: Arch Toxicology. 59. 141-145 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Morio FUKUHARA;Masao KONDO: Toxicology. 39. 165-176 (1986)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Masao Kondo: "Decreased erythrocyte uroporphyrinogen decarboxylase activity in porphyria cutanea tarda" The Japanese Journal of Dermatology. 95. 995-996 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Masao Kondo, Gumpei Urata: "Radiochemical microassay of -aminolevulinic acid dehydratase activity in whole blood and bone marrow" Clinical Chemistry. 31. 427-429 (1985)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Masao Kondo, Taeko Suzuki: "Differences of -aminolevulinate dehydratase activity in the distribution and responses to porphyrogenic compounds in the various tissues of mice" Bulletin of the Institute of Public Health. 35. in press (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Masao Kondo: "High-performance of liquid chromatography of Porphyrins (Review)" LC family. 22. in press (1987)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary
  • [Publications] Masao Kondo, Yoshitaka Shimizu: "The effects of ethanol, estrogen, and hexachlorobenzene on the activities of hepatic -aminolevulinic acid synthase, -aminolevulinic acid dehydratase and uroporphyrinogen decarboxylase in male rats" Arch Toxicology. 59. 141-145 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1986 Final Research Report Summary

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Published: 1987-03-31   Modified: 2016-04-21  

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