| Project/Area Number |
60570485
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| Research Category |
Grant-in-Aid for General Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Research Field |
Radiation science
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| Research Institution | Keio University, School of Medicine, |
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Principal Investigator |
ITO Hisao Department of Radiology, Keio University, School of Medicine., 医学部, 講師 (20095574)
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| Co-Investigator(Kenkyū-buntansha) |
YAMASHITA Shoji Department of Radiology, Keio University, School of Medicine., 医学部放射線医学教室, 兼任講師 (50138103)
NAKAMURA Kayoko Department of Radiology, Keio University, School of Medicine., 医学部放射線医学教室, 助手 (90164285)
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| Project Period (FY) |
1985 – 1986
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| Project Status |
Completed (Fiscal Year 1986)
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| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1986: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1985: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | Radiation Damage of the Lung / WR-2721 / Elastase / Dexamethasone / Survival Rate / Collagen Subtype |
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| Research Abstract |
The thoraxes of 8 week-old female C3H mice wee locally irradiated by X-ray. The survival rates of mice which were given under 11Gy began to decrease 300 days after irradiation and made a slow descent. On the other hand, most mice died until 120 days after exposure when they were exposed to over 15Gy. These results suggest that the mechanisms of death were different between 11-13Gy. When the body weight was checked in both irradiated and non-irradiated groups, those of the former stopped to increase and showed growth retardation, even if a small dose of irradiation was given and the survival rates did not decrease. The determination of body weight in irradiated groups was a good assay method to check the radiation damage.
The protective effects of WR-2721, Elastase and Dexamethasone against radiation damage was determined. WR-2721 was most effective in these. The mice which were given 15Gy died within 120 days after irradiation, however, the survival rate of mice which were given 15Gy an
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d WR-2721 was almost similar as non-irradiated controls at 400 days after irradiation. When body weights of these groups were compared, those of the WR-2721 group were smaller than the non-irradiated control and showed growth retardation. This result also supports that the determination of body weight is more sensitive than the survivla rate to check the radiation damage. Elastase could improve the sirvival rate of irradiated mice when it was combined with 13Gy but it did not work when combined with 15Gy. Elastase might be albe to protect the mice only against the mild radiation damage. Dexamethasone was effective to protect mice against radiation damage even if the drug was given before and 3 months after irradiation. This result is a good information for clinical applications.
The collagen was extracted from the mouse lung with pepsin and acetic acid according to the method by Seyer et al. This method could be available to the mouse lung and the SDS-PAGE analysis showed typical collagen type I petterns. We plan to analyse the collagen subtypes in mice which were treated protective drugs and sacrified several times after irradiation.
The lung lavages and sera from the lung cancer patients were taken before and after irradiation to determine the levels of type <III> procollagen N-terminal peptide in them. The peptide levels in lavage significantly increased after irradiation, especially in patients whose chest X-rays showed radiation fibrosis. Less
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