| Project/Area Number |
60570528
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
内分泌・代謝学
|
|---|
| Research Institution | Kyoto University |
|---|
Principal Investigator |
YAMAMOTO Itsuo Kyoto University, Faculty of Medicine, 医学部, 講師 (60115962)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
YAJIMA Haruaki Kyoto University, Faculty of Pharmacology, 薬学部, 教授 (00025678)
FUKUDA Yoshihiro Kyoto University, Faculty of Medicine, 医学部, 助手 (50127130)
UCHIYAMA Takashi Kyoto University, Faculty of Medicine, 医学部, 助手 (80151900)
|
|---|
| Project Period (FY) |
1985 – 1986
|
| Project Status |
Completed (Fiscal Year 1986)
|
| Budget Amount *help |
¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1986: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1985: ¥1,200,000 (Direct Cost: ¥1,200,000)
|
|---|
| Keywords | Hypercalcemia in Malignancy / Parathyroid Hormone |
|---|
| Research Abstract |
1. Parathyroid hormone (PTH) like substance was purified to an apparent homogeneity from human ovarian tumors, renal cell carcinoma, lung cancer and urinary bladder cancer. The substane was highly basic peptides with approximate molecular weight of 13,000,resorbed mouse bones in vitro and bound to PTH receptor in osteoblastic using cells. Determination of amino-acid sequence in under investigation materials from cell culture medium in which active substances are released.
2. Active vitamin D like substance was identified and purified from both serum and tumor in a lung cancer associated hypercalcemic patient. The active vitamin D like substance is indistinguishable from 1,24-dihydroxyvitamin D3 in its mode of HPLC coloumn elution. This lipid resorbed bone in vitro in similar potency with 1,25-dihydroxyvitamin D3 and circulating concentration of the lipid was high enough to reveal its activity.
3. Bone resorbing substances were fractioned from adult T-cell leukemia cells. The active fractions eluted between 15,000 and 20,000 molecular weights. From the eluting pattern, this substance is recognized to be different from IL-1. In clinical study with ATL patients, bone biopsy was performed and pathological findings was discussed In patient with ATL, bone specimens demonstrated increased number of osteoclasts and absence of tumor cells around osteoclasts suggesting stimulation by humoral factors.
|
